Glioblastoma (GBM) is the most aggressive brain tumor in adults with the highest mortality rate. Despite advances achieved in treatment and research, the median survival for patients with GBM remains <1.5 years. This figure prompted the present study to identify novel genes associated with GBM development and progression to ultimately improve GBM treatment. The current study sought to determine the role of homeobox B3 (HOXB3) in GBM cell invasion and proliferation. HOXB3 was highly expressed in GBM tissues and glioma cell lines. To establish in vitro cell models for investigation, U87-MG and U251-MG, two typical GBM cells, were selected to generate corresponding cells lines that constitutively silenced HOXB3 expression using a lentivirus-mediated RNA interference approach. The results of the knockdown revealed that glioma cells stably expressing HOXB3 short hairpin RNA exhibited significantly decreased proliferation levels when compared with untransfected cells. The effect of HOXB3 on glioma cell invasion was also examined. Silencing of HOXB3 resulted in a marked reduction in invasiveness. Furthermore, HOXB3 silencing led to the upregulation of E-cadherin and downregulation of mesenchymal markers, N-cadherin and vimentin. Taken together, the findings of the present study indicate that HOXB3 promotes cell proliferation and invasion.
Keywords: U251-MG cells; U87-MG cells; homeobox B3.