Disquisition on the interaction of ibuprofen-Zn(II) complex with calf thymus DNA by spectroscopic techniques and the use of Hoechst 33258 and Methylene blue dyes as spectral probes

Nahid Shahabadi; Farshad Shiri; Mohammadsaleh Norouzibazaz; Aliyar Falah

doi:10.1080/15257770.2017.1400048

Disquisition on the interaction of ibuprofen-Zn(II) complex with calf thymus DNA by spectroscopic techniques and the use of Hoechst 33258 and Methylene blue dyes as spectral probes

Nucleosides Nucleotides Nucleic Acids. 2018;37(3):125-146. doi: 10.1080/15257770.2017.1400048. Epub 2018 Feb 20.

Authors

Nahid Shahabadi^{1

2}, Farshad Shiri^{1

3

4}, Mohammadsaleh Norouzibazaz⁵, Aliyar Falah^{6

7}

Affiliations

¹ Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran.
² Medical Biology Research Center (EBRC) Kermanshah University of Medical Sciences, Kermanshah, Iran.
³ Inorganic and Computational Chemistry, Department of Science, Tarbiat Modares University, Tehran, Iran.
⁴ Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.
⁵ Department of Organic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
⁶ Medical Biology Research Center, Arak University of Medical Sciences, Arak, Iran.
⁷ Department of Physical Education, Islamic Azad University, Arak Branch, Arak, Iran.

PMID: 29461923
DOI: 10.1080/15257770.2017.1400048

Abstract

The interaction between ibuprofen-Zn(II) complex and calf thymus DNA in physiological buffer (pH 7.4) was studied with the use of Hoechst 33258 and methylene blue dyes as spectral probes by multi-spectroscopic techniques, and viscosity measurements. It was found that ibuprofen-Zn(II) complex molecules could bind with DNA via groove binding mode as evidenced by: i- DNA binding constant (K_b = (1.00 ± 0.2) × 10⁴ M^-1) from Spectrophotometric studies of the interaction of ibuprofen-Zn(II) complex with DNA is comparable to groove binding drugs. ii- Absorption Spectra of Competitive interaction of ibuprofen-Zn(II) complex and Hoechst 33258 with DNA exhibited the reverse process, The results suggested that interaction of the ibuprofen-Zn(II) complex with calf thymus DNA, is similar to Hoechst 33258 interaction with calf thymus DNA (This was verified by the following fluorescence study). iii- Competitive fluorimetric studies with Hoechst 33258 have shown that ibuprofen-Zn(II) complex exhibit the ability of this complex to displace with DNA-bounded Hoechst 33258, indicating that it binds to DNA in strong competition with Hoechst 33258 for the groove binding. iv- There is no significantly change in the fluorescence intensity of the MB-DNA system upon adding the ibuprofen-Zn(II) complex, indicate that MB molecules are not released from the DNA helix after addition of the ibuprofen-Zn(II) complex and are indicative of a non-intercalative mode of binding. v- Small changes in DNA viscosity in the presence of ibuprofen-Zn(II) complex, indicating weak link to DNA, which is consistent with DNA groove binding. As well as, induced CD spectral changes, and the docking results revealed that groove mechanism is followed by ibuprofen-Zn(II) complex to bind with DNA.

Keywords: DNA interaction; Groove binding; Ibuprofen–Zn(II) complex; Spectral probes; Zn-ibuprofenato.