Time-dependent effect of phytocannabinoid treatments in fat cells

Phytother Res. 2018 Jun;32(6):1080-1089. doi: 10.1002/ptr.6047. Epub 2018 Feb 21.

Abstract

The objectives of this paper is to investigate, demonstrate, and compare the mechanism of action of phytocannabinoids as antidiabetic and anti-obesity agents in preadipocytes and adipocytes, relative to rosiglitazone and metformin. Briefly, cannabis extract, Δ9 -tetrahydrocannabinol and cannabidiol (in very low dosages) were shown to promote glucose uptake higher or to equivalent levels, reduce fat accumulation, and reverse the insulin-resistant state of 3T3-L1 cells more effectively, relative to rosiglitazone and metformin. The phytocannabinoids had a more pronounced effect in preadipocytes undifferentiated model rather than the differentiated model. They induced a protective effect at the mitochondrial level by preventing overactivity of the succinate dehydrogenase pathway (p < .01), unlike rosiglitazone, through activation of the glycerol-3-phosphate dehydrogenase shuttling system. An increase in oxygen consumption and an increased expression of beta to alpha adrenoceptors (p < .05) in treated cells were noted. These findings contribute toward understanding the mechanism of action of phytocannabinoids in fat cells and highlight the antidiabetic and anti-obesity properties of various phytocannabinoids that could potentially support the treatment of obesity-related insulin resistance.

Keywords: beta/alpha adrenoceptors; glycerol-3-phosphate dehydrogenase shuttling system; insulin-resistance; obesity; phytocannabinoids.

MeSH terms

  • Adipocytes / drug effects*
  • Animals
  • Anti-Obesity Agents / pharmacology
  • Anti-Obesity Agents / therapeutic use*
  • Cannabinoids / pharmacology
  • Cannabinoids / therapeutic use*
  • Cannabis
  • Hypoglycemic Agents / pharmacology
  • Hypoglycemic Agents / therapeutic use*
  • Mice
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*

Substances

  • Anti-Obesity Agents
  • Cannabinoids
  • Hypoglycemic Agents
  • Plant Extracts