Polyphenols-rich extract from Pleurotus eryngii with growth inhibitory of HCT116 colon cancer cells and anti-inflammatory function in RAW264.7 cells

Food Funct. 2018 Mar 1;9(3):1601-1611. doi: 10.1039/c7fo01794d. Epub 2018 Feb 21.


Edible mushrooms are rich sources of bioactive components. In this study, a polyphenol-rich extract, designated as PPEP, was isolated from an edible mushroom, Pleurotus eryngii. Using ultra high performance liquid chromatograph combined with triple time-of-flight mass spectrometry (UPLC-TOF/MS/MS), gallic acid monohydrate, 3-(3,4-dihydroxyphenyl)-propionic acid, methyl gallate, syringic acid, ellagic acid and catechin were identified in PPEP. This phenolic-rich extract PPEP exhibited anti-inflammatory effect in lipopolysaccharide-stimulated RAW264.7 macrophages by inhibiting the overproduction of pro-inflammatory mediators including nitric oxide (NO) and reactive oxygen species (ROS). It was demonstrated that the anti-inflammatory effects of PPEP were associated with the inhibition of iNOS expression, suppression of p-IκB protein expression and inhibition of NF-κB and IκB mRNA expression. Next, the inhibitory effect of PPEP against human colon cancer cells was also determined. PPEP suppressed cell proliferation of human colon cancer HCT116 cells in a dose- and time-dependent fashion, while it showed no inhibitory effect on normal human colonic myofibroblasts CCD-18Co cells at the same tested concentrations (0-200 μg mL-1). Moreover, PPEP induced cell cycle arrest and led to extensive cellular apoptosis in human colon cancer cells, which was associated with the downregulation of cell cycle-related signaling protein, e.g. cyclin B and cyclin E, and the upregulation of apoptosis-related signaling protein caspase-3 and cleaved caspase-3. Overall, our results provided a basis for using PPEP as a promising preventive agent against inflammatory disease and colon cancer.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Cycle / drug effects
  • Cell Proliferation / drug effects
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / immunology
  • Colonic Neoplasms / physiopathology*
  • Growth Inhibitors / chemistry
  • Growth Inhibitors / pharmacology*
  • HCT116 Cells
  • Humans
  • Macrophages / drug effects
  • Macrophages / immunology
  • Mice
  • NF-kappa B / genetics
  • NF-kappa B / immunology
  • Nitric Oxide / immunology
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Pleurotus / chemistry*
  • Polyphenols / chemistry
  • Polyphenols / pharmacology*
  • RAW 264.7 Cells
  • Reactive Oxygen Species / immunology
  • Tandem Mass Spectrometry


  • Anti-Inflammatory Agents
  • Growth Inhibitors
  • NF-kappa B
  • Plant Extracts
  • Polyphenols
  • Reactive Oxygen Species
  • Nitric Oxide