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, 555 (7695), 197-203

The Genomic History of Southeastern Europe

Iain Mathieson  1 Songül Alpaslan-Roodenberg  1 Cosimo Posth  2   3 Anna Szécsényi-Nagy  4 Nadin Rohland  1 Swapan Mallick  1   5 Iñigo Olalde  1 Nasreen Broomandkhoshbacht  1   5 Francesca Candilio  6 Olivia Cheronet  6   7 Daniel Fernandes  6   8 Matthew Ferry  1   5 Beatriz Gamarra  6 Gloria González Fortes  9 Wolfgang Haak  2   10 Eadaoin Harney  1   5 Eppie Jones  11   12 Denise Keating  6 Ben Krause-Kyora  2 Isil Kucukkalipci  3 Megan Michel  1   5 Alissa Mittnik  2   3 Kathrin Nägele  2 Mario Novak  6   13 Jonas Oppenheimer  1   5 Nick Patterson  14 Saskia Pfrengle  3 Kendra Sirak  6   15 Kristin Stewardson  1   5 Stefania Vai  16 Stefan Alexandrov  17 Kurt W Alt  18   19   20 Radian Andreescu  21 Dragana Antonović  22 Abigail Ash  6 Nadezhda Atanassova  23 Krum Bacvarov  17 Mende Balázs Gusztáv  4 Hervé Bocherens  24   25 Michael Bolus  26 Adina Boroneanţ  27 Yavor Boyadzhiev  17 Alicja Budnik  28 Josip Burmaz  29 Stefan Chohadzhiev  30 Nicholas J Conard  25   31 Richard Cottiaux  32 Maja Čuka  33 Christophe Cupillard  34   35 Dorothée G Drucker  25 Nedko Elenski  36 Michael Francken  37 Borislava Galabova  38 Georgi Ganetsovski  39 Bernard Gély  40 Tamás Hajdu  41 Veneta Handzhyiska  42 Katerina Harvati  25   37 Thomas Higham  43 Stanislav Iliev  44 Ivor Janković  13   45 Ivor Karavanić  45   46 Douglas J Kennett  47 Darko Komšo  33 Alexandra Kozak  48 Damian Labuda  49 Martina Lari  16 Catalin Lazar  21   50 Maleen Leppek  51 Krassimir Leshtakov  42 Domenico Lo Vetro  52   53 Dženi Los  29 Ivaylo Lozanov  42 Maria Malina  26 Fabio Martini  52   53 Kath McSweeney  54 Harald Meller  20 Marko Menđušić  55 Pavel Mirea  56 Vyacheslav Moiseyev  57 Vanya Petrova  42 T Douglas Price  58 Angela Simalcsik  59 Luca Sineo  60 Mario Šlaus  61 Vladimir Slavchev  62 Petar Stanev  36 Andrej Starović  63 Tamás Szeniczey  41 Sahra Talamo  64 Maria Teschler-Nicola  7   65 Corinne Thevenet  32 Ivan Valchev  42 Frédérique Valentin  66 Sergey Vasilyev  67 Fanica Veljanovska  68 Svetlana Venelinova  69 Elizaveta Veselovskaya  67 Bence Viola  70   71 Cristian Virag  72 Joško Zaninović  73 Steve Zäuner  74 Philipp W Stockhammer  2   51 Giulio Catalano  60 Raiko Krauß  75 David Caramelli  16 Gunita Zariņa  76 Bisserka Gaydarska  77 Malcolm Lillie  78 Alexey G Nikitin  79 Inna Potekhina  48 Anastasia Papathanasiou  80 Dušan Borić  81 Clive Bonsall  54 Johannes Krause  2   3 Ron Pinhasi  6   7 David Reich  1   5   14
Affiliations

The Genomic History of Southeastern Europe

Iain Mathieson et al. Nature.

Abstract

Farming was first introduced to Europe in the mid-seventh millennium bc, and was associated with migrants from Anatolia who settled in the southeast before spreading throughout Europe. Here, to understand the dynamics of this process, we analysed genome-wide ancient DNA data from 225 individuals who lived in southeastern Europe and surrounding regions between 12000 and 500 bc. We document a west-east cline of ancestry in indigenous hunter-gatherers and, in eastern Europe, the early stages in the formation of Bronze Age steppe ancestry. We show that the first farmers of northern and western Europe dispersed through southeastern Europe with limited hunter-gatherer admixture, but that some early groups in the southeast mixed extensively with hunter-gatherers without the sex-biased admixture that prevailed later in the north and west. We also show that southeastern Europe continued to be a nexus between east and west after the arrival of farmers, with intermittent genetic contact with steppe populations occurring up to 2,000 years earlier than the migrations from the steppe that ultimately replaced much of the population of northern Europe.

Figures

Extended Data Figure 1
Extended Data Figure 1
PCA of 486 ancient individuals, projected onto principal components defined by 777 present-day West Eurasian individuals (grey points). This differs from Figure 1B in that the plot is not cropped and the present-day individuals are shown.
Extended Data Figure 2
Extended Data Figure 2
Supervised ADMIXTURE analysis modeling each ancient individual (one per row), as a mixture of populations represented by clusters that are constrained to contain Anatolian Neolithic (grey), Yamnaya from Samara (yellow), EHG (pink) and WHG (green) populations. Dates in parentheses indicate approximate range of individuals in each population. This differs from Figure 1D in that it contains some previously published samples, and includes sample IDs.
Extended Data Figure 3
Extended Data Figure 3
Unsupervised ADMIXTURE plot from k=4 to 12, on a dataset consisting of 1099 present-day individuals and 476 ancient individuals. We show newly reported ancient individuals and some previously published individuals for comparison.
Extended Data Figure 4
Extended Data Figure 4
Spatial structure in hunter-gatherers. Estimated effective migration surface (EEMS). This fits a model of genetic relatedness where individuals move (in a random direction) from generation to generation on an underlying grid so that genetic relatedness is determined by distance. The migration parameter m defines the local rate of migration, varies on the grid and is inferred. This plot shows log10(m), scaled relative to the average migration rate (which is arbitrary). Thus log10(m)=2, for example, implies that the rate of migration at this point on the grid is 100 times higher than average. To restrict as much as possible to hunter-gatherer structure, the migration surface is inferred using data from 116 individuals that date to earlier than ~5000 BCE and have no NW Anatolian-related ancestry. Though the migration surface is sensitive to sampling, and fine-scale features may not be interpretable, the migration “barrier” (region of low migration) running north-south and separating populations with primarily WHG from primarily EHG ancestry seems to be robust, and consistent with inferred admixture proportions. This analysis suggests that Mesolithic hunter-gatherer population structure was clustered and not smoothly clinal, in the sense that genetic differentiation did not vary consistently with distance. Superimposed on this background, pies show the WHG, EHG and CHG ancestry proportions inferred for populations used to construct the migration surface (another way of visualizing the data in Figure 2, Supplementary Table 3.1.3 – we use two population models if they fit with p>0.01, and three population models otherwise). Pies with only a single color are those that were fixed to be the source populations.
Extended Data Figure 5
Extended Data Figure 5
log-likelihood surfaces for the proportion of female (x-axis) and male (y-axis) ancestors that are hunter-gatherer-related for the combined populations analyzed in Figure 3C, and the two populations with the strongest evidence for sex-bias. Numbers in parentheses give the number of individuals in each group. Log-likelihood scale ranges from 0 to -10, where 0 is the feasible point with the highest likelihood.
Figure 1
Figure 1. Geographic and genetic structure of 216 newly reported individuals
A: Locations of newly reported individuals. B: Ancient individuals projected onto principal components defined by 777 present-day West Eurasians (shown in Extended Data Figure 1). Includes selected published individuals (faded circles, labeled) and newly reported individuals (other symbols, outliers enclosed in black circles). Colored polygons cover individuals that had cluster memberships fixed at 100% for supervised admixture analysis. C: Date (direct or contextual) for each sample and approximate chronology of southeastern Europe. D: Supervised ADMIXTURE analysis, modeling each ancient individual (one per row), as a mixture of population clusters constrained to contain Anatolian Neolithic (grey), Yamnaya from Samara (yellow), EHG (pink) and WHG (green) populations. Dates in parentheses indicate approximate range of individuals in each population. See Extended Data Figure 2 for individual sample IDs. Map data in A from the R package maps.
Figure 2
Figure 2. Structure and change in hunter-gatherer-related populations
Inferred ancestry proportions for populations modeled as a mixture of WHG, EHG and CHG (Supplementary Table S3.1.3). Dashed lines show populations from the same geographic region. Percentages indicate proportion of WHG+EHG ancestry. Standard errors range from 1.5-8.3% (Supplementary Table S3.1.3).
Figure 3
Figure 3. Structure and change in NW Anatolian Neolithic-related populations
A: Populations modeled as a mixture of NW Anatolia Neolithic, WHG, and EHG. Dashed lines show temporal relationships between populations from the same geographic region. Percentages indicate proportion of WHG+EHG ancestry. Standard errors range from 0.7-6.0% (Supplementary Table S3.2.2). B: Z-scores for the difference in hunter-gatherer-related ancestry on the autosomes compared to the X chromosome when populations are modeled as a mixture of NW Anatolia Neolithic and WHG (N=126 individuals, group sizes in parentheses). Positive values indicate more hunter-gatherer-related ancestry on the autosomes and thus male-biased hunter-gatherer ancestry. “Combined” populations merge all individuals from different times from a geographic area. C: Hunter-gatherer-related ancestry proportions on the autosomes, X chromosome, mitochondrial DNA (i.e. mt haplogroup U), and the Y chromosome (i.e. Y chromosome haplogroups I2, R1 and C1). Points show qpAdm (autosomes and X chromosome) or maximum likelihood (MT and Y chromosome) estimates and bars show approximate 95% confidence intervals (N=109 individuals, group sizes in parentheses).

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References

    1. Tringham RE. In: The Transition to Agriculture in Prehistoric Europe. Price D, editor. Cambridge University Press; 2000. pp. 19–56.
    1. Bellwood P. First Farmers: The Origins of Agricultural Societies. 2nd. Wiley-Blackwell; 2004.
    1. Golitko M. In: Ancient Europe, 8000 BC to AD 1000: An Encyclopedia of the Barbarian World. Bogucki P, Crabtree PJ, editors. Charles Scribners & Sons; 2003. pp. 259–266.
    1. Vander Linden M. In: Investigating Archaeological Cultures: Material Culture, Variability, and Transmission. Roberts BW, Linden M Vander, editors. Springer; 2012. pp. 289–319.
    1. Bramanti B, et al. Genetic discontinuity between local hunter-gatherers and central Europe’s first farmers. Science. 2009;326:137–140. - PubMed

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