Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts

Masahiro Kuragano; Taro Q P Uyeda; Keiju Kamijo; Yota Murakami; Masayuki Takahashi

doi:10.1091/mbc.E17-04-0215

Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts

Mol Biol Cell. 2018 Apr 15;29(8):911-922. doi: 10.1091/mbc.E17-04-0215. Epub 2018 Mar 23.

Authors

Masahiro Kuragano¹, Taro Q P Uyeda², Keiju Kamijo³, Yota Murakami^{1

4}, Masayuki Takahashi^{1

4}

Affiliations

¹ Graduate School of Chemical Science and Engineering, Hokkaido University, Sapporo, Hokkaido 060-8628, Japan.
² Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
³ Department of Anatomy, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi 981-8558, Japan.
⁴ Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.

Abstract

We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation.

Publication types

Research Support, Non-U.S. Gov't
Video-Audio Media

MeSH terms

Cell Line
Cell Movement*
Cell Polarity
Cell Shape
Fibroblasts / cytology*
Gene Knockdown Techniques
Humans
Nonmuscle Myosin Type IIA / metabolism*
Nonmuscle Myosin Type IIB / metabolism*
Phosphorylation
Stress Fibers / metabolism*

Substances

Nonmuscle Myosin Type IIA
Nonmuscle Myosin Type IIB