Objective: To explore the value of automatic photography in the observation of results of Schistosoma japonicum miracidium hatching experiments.
Methods: Some fresh S. japonicum eggs were added into cow feces, and the samples of feces were divided into a low infested experimental group and a high infested group (40 samples each group). In addition, there was a negative control group with 40 samples of cow feces without S. japonicum eggs. The conventional nylon bag S. japonicum miracidium hatching experiments were performed. The process was observed with the method of flashlight and magnifying glass combined with automatic video (automatic photography method), and, at the same time, with the naked eye observation method. The results were compared.
Results: In the low infested group, the miracidium positive detection rates were 57.5% and 85.0% by the naked eye observation method and automatic photography method, respectively (χ2 = 11.723, P < 0.05). In the high infested group, the positive detection rates were 97.5% and 100% by the naked eye observation method and automatic photography method, respectively (χ2 = 1.253, P > 0.05). In the two infested groups, the average positive detection rates were 77.5% and 92.5% by the naked eye observation method and automatic photography method, respectively (χ2 = 6.894, P < 0.05).
Conclusions: The automatic photography can effectively improve the positive detection rate in the S. japonicum miracidium hatching experiments.
［摘要］目的 探讨自动摄影术用于观察常规尼龙绢集卵毛蚴孵化实验结果的价值。方法 在牛粪便中掺入一定量的 血吸虫新鲜虫卵, 以含虫卵的粪便为实验组, 包括低污染组和高污染组; 以不含虫卵的粪便为阴性对照组, 每组40份样 本。以常规血吸虫尼龙绢集卵孵化法对粪便样本进行毛蚴孵化, 采用手电筒光照、放大镜及自动录像相结合的方法, 对 粪便样本中毛蚴孵化的过程进行录像。通过观察录像记录, 判断粪便样本中是否有血吸虫毛蚴孵出。同时采用常规肉 眼观察方法进行结果判断, 比较自动摄影术与肉眼观察法2种方法的毛蚴阳性检出率差异及优缺点。结果建立了手 电筒光照、放大镜影像放大及自动摄影术相结合的毛蚴孵化结果观察方法, 证实了自动摄影术对粪便中血吸虫虫卵毛蚴 孵化的阳性检出率高于常规肉眼观察方法, 尤其是在虫卵低污染组检出率有明显的差异。40份低污染组中常规肉眼观 察法和自动摄影术观察法的阳性检出率分别为57.5%和85.0% (χ2 = 11.723, P < 0.05), 40份高污染组中阳性检出率分别 为97.5%和100% (χ2 = 1.253, P > 0.05), 80份高低污染组平均阳性检出率分别为77.5%和92.5% (χ2 = 6.894, P < 0.05)。结论 自动摄影术可有效提高毛蚴常规尼龙绢集卵孵化实验的阳性检出率。.
Keywords: Automatic photography; Hatching experiment with the nylon bag; Miracidium; Schistosoma japonicum.