Fish mutant, where is thy phenotype?

doi:10.1371/journal.pgen.1007197

Comment

. 2018 Feb 22;14(2):e1007197.

doi: 10.1371/journal.pgen.1007197. eCollection 2018 Feb.

Fish mutant, where is thy phenotype?

Darius Balciunas¹

Affiliations

PMID: 29470494
PMCID: PMC5823365
DOI: 10.1371/journal.pgen.1007197

Comment

Fish mutant, where is thy phenotype?

Darius Balciunas. PLoS Genet. 2018.

. 2018 Feb 22;14(2):e1007197.

doi: 10.1371/journal.pgen.1007197. eCollection 2018 Feb.

Author

Darius Balciunas¹

Affiliation

¹ Department of Biology, Temple University, Philadelphia, Pennsylvania, United States of America.

PMID: 29470494
PMCID: PMC5823365
DOI: 10.1371/journal.pgen.1007197

No abstract available

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Conflict of interest statement

The author has declared that no competing interests exist.

Figures

**Fig 1. Reverse genetics and mutant phenotypes.**
Green panels represent a scenario where an engineered mutant displays a phenotype. In the absence of a phenotype (red panel), the possibilities of genetic compensation (blue panels) or incomplete loss of function (yellow panels) should be considered. If incomplete loss of function is likely, this information could be used to engineer a better allele (purple arrow). In mutants without overt phenotypes, more specific or more stringent assays may be needed to reveal a phenotype (grey arrow). In addition to scenarios displayed in the figure, genetic redundancy is known to lead to an absence of mutant phenotypes.

See this image and copyright information in PMC

Comment on

mRNA processing in mutant zebrafish lines generated by chemical and CRISPR-mediated mutagenesis produces unexpected transcripts that escape nonsense-mediated decay.
Anderson JL, Mulligan TS, Shen MC, Wang H, Scahill CM, Tan FJ, Du SJ, Busch-Nentwich EM, Farber SA. Anderson JL, et al. PLoS Genet. 2017 Nov 21;13(11):e1007105. doi: 10.1371/journal.pgen.1007105. eCollection 2017 Nov. PLoS Genet. 2017. PMID: 29161261 Free PMC article.

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References

1. Sjoberg EA. Logical fallacies in animal model research. Behav Brain Funct. 2017;13(1):3 doi: 10.1186/s12993-017-0121-8 ; PubMed Central PMCID: PMCPMC5312558. - DOI - PMC - PubMed
1. Jao LE, Wente SR, Chen WB. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(34):13904–9. doi: 10.1073/pnas.1308335110 PubMed PMID: WOS:000323271400055. - DOI - PMC - PubMed
1. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature biotechnology. 2013;31(3):227–9. doi: 10.1038/nbt.2501 ; PubMed Central PMCID: PMC3686313. - DOI - PMC - PubMed
1. Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE. 2014;9(5):e98186 doi: 10.1371/journal.pone.0098186 ; PubMed Central PMCID: PMC4038517. - DOI - PMC - PubMed
1. Nasevicius A, Ekker SC. Effective targeted gene 'knockdown' in zebrafish. Nature genetics. 2000;26(2):216–20. doi: 10.1038/79951 . - DOI - PubMed

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[1] Sjoberg EA. Logical fallacies in animal model research. Behav Brain Funct. 2017;13(1):3 doi: 10.1186/s12993-017-0121-8 ; PubMed Central PMCID: PMCPMC5312558. - DOI - PMC - PubMed

[2] Sjoberg EA. Logical fallacies in animal model research. Behav Brain Funct. 2017;13(1):3 doi: 10.1186/s12993-017-0121-8 ; PubMed Central PMCID: PMCPMC5312558. - DOI - PMC - PubMed

[3] Jao LE, Wente SR, Chen WB. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(34):13904–9. doi: 10.1073/pnas.1308335110 PubMed PMID: WOS:000323271400055. - DOI - PMC - PubMed

[4] Jao LE, Wente SR, Chen WB. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(34):13904–9. doi: 10.1073/pnas.1308335110 PubMed PMID: WOS:000323271400055. - DOI - PMC - PubMed

[5] Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature biotechnology. 2013;31(3):227–9. doi: 10.1038/nbt.2501 ; PubMed Central PMCID: PMC3686313. - DOI - PMC - PubMed

[6] Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature biotechnology. 2013;31(3):227–9. doi: 10.1038/nbt.2501 ; PubMed Central PMCID: PMC3686313. - DOI - PMC - PubMed

[7] Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE. 2014;9(5):e98186 doi: 10.1371/journal.pone.0098186 ; PubMed Central PMCID: PMC4038517. - DOI - PMC - PubMed

[8] Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE. 2014;9(5):e98186 doi: 10.1371/journal.pone.0098186 ; PubMed Central PMCID: PMC4038517. - DOI - PMC - PubMed

[9] Nasevicius A, Ekker SC. Effective targeted gene 'knockdown' in zebrafish. Nature genetics. 2000;26(2):216–20. doi: 10.1038/79951 . - DOI - PubMed

[10] Nasevicius A, Ekker SC. Effective targeted gene 'knockdown' in zebrafish. Nature genetics. 2000;26(2):216–20. doi: 10.1038/79951 . - DOI - PubMed

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Fish mutant, where is thy phenotype?

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Fish mutant, where is thy phenotype?

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