mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy

Sci Rep. 2018 Feb 23;8(1):3568. doi: 10.1038/s41598-018-21656-8.

Abstract

Cortical dysplasia (CD) is a common cause for intractable epilepsy. Hyperactivation of the mechanistic target of rapamycin (mTOR) pathway has been implicated in CD; however, the mechanisms by which mTOR hyperactivation contribute to the epilepsy phenotype remain elusive. Here, we investigated whether constitutive mTOR hyperactivation in the hippocampus is associated with altered voltage-gated ion channel expression in the neuronal subset-specific Pten knockout (NS-Pten KO) mouse model of CD with epilepsy. We found that the protein levels of Kv1.1, but not Kv1.2, Kv1.4, or Kvβ2, potassium channel subunits were increased, along with altered Kv1.1 distribution, within the hippocampus of NS-Pten KO mice. The aberrant Kv1.1 protein levels were present in young adult (≥postnatal week 6) but not juvenile (≤postnatal week 4) NS-Pten KO mice. No changes in hippocampal Kv1.1 mRNA levels were found between NS-Pten KO and WT mice. Interestingly, mTOR inhibition with rapamycin treatment at early and late stages of the pathology normalized Kv1.1 protein levels in NS-Pten KO mice to WT levels. Together, these studies demonstrate altered Kv1.1 protein expression in association with mTOR hyperactivation in NS-Pten KO mice and suggest a role for mTOR signaling in the modulation of voltage-gated ion channel expression in this model.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Epilepsy / complications
  • Epilepsy / genetics*
  • Epilepsy / pathology
  • Gene Expression Regulation / drug effects
  • Hippocampus / metabolism
  • Hippocampus / pathology
  • Humans
  • Kv1.1 Potassium Channel / genetics*
  • Kv1.2 Potassium Channel / genetics
  • Kv1.4 Potassium Channel / genetics
  • Malformations of Cortical Development / complications
  • Malformations of Cortical Development / genetics*
  • Malformations of Cortical Development / pathology
  • Mice
  • Mice, Knockout
  • PTEN Phosphohydrolase / genetics*
  • Signal Transduction / drug effects
  • Sirolimus / administration & dosage
  • TOR Serine-Threonine Kinases / antagonists & inhibitors
  • TOR Serine-Threonine Kinases / genetics*

Substances

  • Kv1.2 Potassium Channel
  • Kv1.4 Potassium Channel
  • Kv1.1 Potassium Channel
  • TOR Serine-Threonine Kinases
  • mTOR protein, mouse
  • PTEN Phosphohydrolase
  • Pten protein, mouse
  • Sirolimus