Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis

Methods Mol Biol. 2018;1732:477-493. doi: 10.1007/978-1-4939-7598-3_30.

Abstract

Macrophages are a driving force in the development and progression of atherosclerosis, a chronic condition that can lead to cardiovascular disease. In this chapter we describe methods that monitor macrophage cholesterol homeostasis such as cholesterol synthesis, uptake, and efflux, all with the use of AMPK activators and potential genetic models that could help shed light on the role of this metabolic regulator in atherosclerosis and other chronic diseases.

Keywords: AMP-activated protein kinase; Atherosclerosis; Cholesterol homeostasis; Macrophages; Reverse cholesterol transport.

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Animals
  • Atherosclerosis / pathology
  • Cell Line
  • Cholesterol / metabolism*
  • Culture Media, Conditioned
  • Enzyme Activation / drug effects*
  • Enzyme Activators / pharmacology*
  • Lipid Metabolism / drug effects
  • Macrophages / metabolism*
  • Primary Cell Culture

Substances

  • Culture Media, Conditioned
  • Enzyme Activators
  • Cholesterol
  • AMP-Activated Protein Kinases