Abstract
Astragaloside IV (AS-IV) has been reported to possess anti-metastasis activity in cancer cells. However, it is unknown whether AS-IV could inhibit epithelial-mesenchymal transition (EMT), a cellular de-differentiation program that promotes metastasis, in cancer cells. The aim of this study was to study the effect and mechanism of AS-IV on EMT in gastric cancer (GC) cells. The results showed that AS-IV significantly inhibited cell viability, invasion, and migration of GC cells. The E-cadherin to N-cadherin switch and expression of Vimentin and metastasis-related genes were induced by transforming growth factor β1 (TGF-β1), whereas AS-IV reversed the induction. In addition, AS-IV inhibited TGF-β1-induced activation of PI3K/Akt/NF-κB. Inhibition of the PI3K/Akt/NF-κB pathway reversed TGF-β1-induced EMT. In conclusion, AS-IV inhibited TGF-β1-induced EMT through inhibition of the PI3K/Akt/NF-κB pathway in GC cells. AS-IV might be an effective candidate for the treatment for GC.
Keywords:
NF-κB; PI3K/Akt; astragaloside IV; epithelial-mesenchymal transition; gastric cancer.
Copyright © 2018 John Wiley & Sons, Ltd.
MeSH terms
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Antigens, CD
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Cadherins / genetics
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Cadherins / metabolism
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Cell Line, Tumor
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Cell Movement / drug effects
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Cell Movement / genetics
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Cell Survival / drug effects
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Cell Survival / genetics
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Down-Regulation / drug effects
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Down-Regulation / genetics
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Epithelial-Mesenchymal Transition / drug effects*
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Epithelial-Mesenchymal Transition / genetics
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Phosphatidylinositol 3-Kinases / genetics
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Phosphatidylinositol 3-Kinases / metabolism
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Proto-Oncogene Proteins c-akt / genetics
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Proto-Oncogene Proteins c-akt / metabolism
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Saponins / pharmacology*
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Stomach Neoplasms / genetics
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology*
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Transcription Factor RelA / genetics
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Transcription Factor RelA / metabolism
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Transforming Growth Factor beta1 / antagonists & inhibitors
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Transforming Growth Factor beta1 / metabolism
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Transforming Growth Factor beta1 / pharmacology*
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Triterpenes / pharmacology*
Substances
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Antigens, CD
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CDH1 protein, human
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Cadherins
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NF-kappa B
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RELA protein, human
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Saponins
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Transcription Factor RelA
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Transforming Growth Factor beta1
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Triterpenes
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astragaloside A
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Phosphatidylinositol 3-Kinases
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Proto-Oncogene Proteins c-akt