Abstract
Aims:
To investigate the roles of Lats1/p-YAP1 pathway in TBI-induced neuronal apoptosis and neurological deficits in rats.
Results:
We found that Lats1 and YAP1 were expressed in cerebral cortex neurons of Sprague-Dawley rats, and the phosphorylation levels of Lats1 and YAP1 in injured regions were significantly increased after TBI. Furthermore, inhibition of Lats1 not only decreased the level of p-YAP1, but also attenuated neuronal apoptosis and neurological impairment.
Conclusions:
Our work demonstrates that inhibition of Lats1/p-YAP1 pathway mitigates neuronal apoptosis and neurological deficits in a rat model of TBI.
Keywords:
Lats1; YAP1; neurological deficit; neuronal apoptosis; traumatic brain injury.
© 2018 John Wiley & Sons Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Apoptosis / physiology*
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism*
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Blood-Brain Barrier / drug effects
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Blood-Brain Barrier / pathology
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Brain Edema / etiology
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Brain Edema / pathology
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Brain Injuries, Traumatic / complications*
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Brain Injuries, Traumatic / pathology*
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Caspase 3 / metabolism
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Disease Models, Animal
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Fluoresceins / metabolism
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In Situ Nick-End Labeling
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Male
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Nervous System Diseases / etiology*
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Neurons / drug effects
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Neurons / pathology*
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Neurons / physiology
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Phosphopyruvate Hydratase / metabolism
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Phosphorylation / drug effects
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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RNA, Small Interfering / therapeutic use
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Rats
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Rats, Sprague-Dawley
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Signal Detection, Psychological / drug effects
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Signal Detection, Psychological / physiology*
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Time Factors
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YAP-Signaling Proteins
Substances
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Apoptosis Regulatory Proteins
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Fluoresceins
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RNA, Small Interfering
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YAP-Signaling Proteins
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Yap1 protein, rat
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fluoro jade
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Lats1 protein, rat
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Protein Serine-Threonine Kinases
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Caspase 3
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Phosphopyruvate Hydratase