Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology

Genome Biol. 2018 Feb 28;19(1):27. doi: 10.1186/s13059-018-1409-1.

Abstract

The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient process. A new study shows that the in situ delivery of CRISPR/Cas9 reagents into pregnant mice results in a high efficiency of editing, and enables the rapid generation of both simple and complex alleles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Comment

MeSH terms

  • Alleles
  • Animals
  • CRISPR-Cas Systems
  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • Gene Editing*
  • Gene Transfer Techniques
  • Mice
  • Ribonucleoproteins

Substances

  • Ribonucleoproteins