Impacts of fullerene C60 and virgin olive oil on cadmium-induced genotoxicity in rats

Abstract

Currently, cadmium is considered to be one of the major environmental pollutants. Environmentally, cadmium is released in various forms e.g. oxide, chloride and sulphide. The aim of the present study was to examine the genotoxic impact of fullerene nanoparticles C₆₀ (C₆₀) and virgin olive oil (VOO) on cadmium chloride (CdCl₂)-induced genotoxicity in rats. To evaluate these effects on DNA damage and chromosomal frequency, 25 albino rats were randomly assigned to 5 groups (n=5 per group): Group 1 served as a control; Group 2 received a single intraperitoneal dose of CdCl₂ (3.5mg/kg); Group 3 animals were treated with C₆₀ (4mg/kg, orally) every other day for 20days; Group 4 received a single intraperitoneal dose of CdCl₂ (3.5mg/kg) and an oral dose of C₆₀ (4mg/kg); and Group 5 received a single intraperitoneal dose of CdCl₂ (3.5mg/kg) and oral doses of VOO every other day for 20 consecutive days. Genotoxic and anti-genotoxic effects of C₆₀ and VOO were evaluated in the liver, kidney and bone marrow using molecular and cytogenetic assays. As expected, CdCl₂ and C₆₀ administration was associated with band number alterations in both liver and kidney; however, C₆₀ pretreatment recovered to approximately basal number. Surprisingly, C₆₀ and VOO significantly attenuated the genotoxic effects caused by CdCl₂ in livers and kidneys. In bone marrow, in addition to a reduction in the chromosomal number, several chromosomal aberrations were caused by CdCl₂. These chromosomal alterations were also reversed by C₆₀ and VOO. In conclusion, molecular and cytogenetic studies showed that C₆₀ and VOO exhibit anti-genotoxic agents against CdCl₂-induced genotoxicity in rats. Further studies are needed to investigate the optimal conditions for potential biomedical applications of these anti-genotoxic agents.

Keywords: Cadmium chloride; DNA damage and chromosomal aberration; Fullerene C(60); Genotoxicity.