Downregulation of MicroRNA-455-3p Links to Proliferation and Drug Resistance of Pancreatic Cancer Cells via Targeting TAZ

Ting Zhan; Xiaodong Huang; Xia Tian; Xiaoli Chen; Yu Ding; Hesheng Luo; Yadong Zhang

doi:10.1016/j.omtn.2017.12.002

Downregulation of MicroRNA-455-3p Links to Proliferation and Drug Resistance of Pancreatic Cancer Cells via Targeting TAZ

Mol Ther Nucleic Acids. 2018 Mar 2:10:215-226. doi: 10.1016/j.omtn.2017.12.002. Epub 2017 Dec 9.

Authors

Ting Zhan¹, Xiaodong Huang², Xia Tian², Xiaoli Chen², Yu Ding³, Hesheng Luo⁴, Yadong Zhang⁵

Affiliations

¹ Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, China; Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China; Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
² Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, China.
³ Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.
⁴ Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
⁵ Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China. Electronic address: yadong-zhang@hotmail.com.

Abstract

Drug resistance is a major cause of treatment failure in pancreatic cancer. The limited evidence indicates the involvement of miR-455-3p in chemotherapy resistance of cancer. Here we observed by qPCR that miR-455-3p was significantly decreased in pancreatic cancer tissues and cell lines. We then confirmed that the inhibition of miR-455-3p increased cell proliferation and gemcitabine resistance of pancreatic cancer, whereas forced overexpression of miR-455-3p had the opposite effect. Furthermore, we demonstrated that TAZ, which is associated with drug resistance of pancreatic cancer, is a new direct downstream target of miR-455-3p. Our present study suggests that miR-455-3p contributes to cell proliferation and drug resistance in pancreatic cancer cells via targeting TAZ.

Keywords: TAZ; drug resistance; gemcitabine; miR-455-3p; pancreatic cancer.