Resident T Cells in Resolved Psoriasis Steer Tissue Responses that Stratify Clinical Outcome

J Invest Dermatol. 2018 Aug;138(8):1754-1763. doi: 10.1016/j.jid.2018.02.030. Epub 2018 Mar 3.


Psoriasis is driven by focal disruptions of the immune-homeostasis in human skin. Local relapse following cessation of therapy is common and unpredictable, which complicates clinical management of psoriasis. We have previously shown that pathogenic resident T cells accumulate in active and resolved psoriasis, but whether these cells drive psoriasiform tissue reactions is less clear. Here, we activated T cells within skin explants using the pan-T cell activating antibody OKT-3. To explore if T cells induced different tissue response patterns in healthy and psoriasis afflicted skin, transcriptomic analyses were performed with RNA-sequencing and Nanostring. Core tissue responses dominated by IFN-induced pathways were triggered regardless of the inflammatory status of the skin. In contrast, pathways induced by IL-17A, including Defensin beta 2 and keratinocyte differentiation markers, were activated in psoriasis samples. An integrated analysis of IL-17A and IFN-related responses revealed that IL-17 dominated tissue response correlated with early relapse following UVB treatment. Stratification of tissue responses to T cell activation in resolved lesions could potentially offer individualized prediction of disease relapse during long-term immunomodulatory treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biopsy
  • Cells, Cultured
  • Female
  • Follow-Up Studies
  • Gene Expression Profiling
  • Humans
  • Immunologic Memory / radiation effects*
  • Interleukin-17 / immunology
  • Interleukin-17 / metabolism
  • Keratinocytes / immunology
  • Keratinocytes / radiation effects
  • Lymphocyte Activation / radiation effects*
  • Male
  • Middle Aged
  • Muromonab-CD3 / immunology
  • Psoriasis / immunology*
  • Psoriasis / pathology
  • Psoriasis / radiotherapy
  • Recurrence
  • Sequence Analysis, RNA
  • Skin / cytology
  • Skin / immunology
  • Skin / pathology
  • Skin / radiation effects
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / radiation effects
  • Tissue Culture Techniques
  • Treatment Outcome
  • Ultraviolet Therapy / methods*
  • beta-Defensins / immunology
  • beta-Defensins / metabolism


  • DEFB4A protein, human
  • IL17A protein, human
  • Interleukin-17
  • Muromonab-CD3
  • beta-Defensins