The chaperone protein HSP47: a platelet collagen binding protein that contributes to thrombosis and hemostasis

J Thromb Haemost. 2018 May;16(5):946-959. doi: 10.1111/jth.13998. Epub 2018 Apr 15.

Abstract

Essentials Heat shock protein 47 (HSP47), a collagen specific chaperone is present on the platelet surface. Collagen mediated platelet function was reduced following blockade or deletion of HSP47. GPVI receptor regulated signalling was reduced in HSP47 deficient platelets. Platelet HSP47 tethers to exposed collagen thus modulating thrombosis and hemostasis.

Summary: Objective Heat shock protein 47 (HSP47) is an intracellular chaperone protein that is vital for collagen biosynthesis in collagen secreting cells. This protein has also been shown to be present on the surface of platelets. Given the importance of collagen and its interactions with platelets in triggering hemostasis and thrombosis, in this study we sought to characterize the role of HSP47 in these cells. Methods and Results The deletion of HSP47 in mouse platelets or its inhibition in human platelets reduced their function in response to collagen and the GPVI agonist (CRP-XL), but responses to thrombin were unaltered. In the absence of functional HSP47, the interaction of collagen with platelets was reduced, and this was associated with reduced GPVI-collagen binding, signalling and platelet activation. Thrombus formation on collagen, under arterial flow conditions, was also decreased following the inhibition or deletion of HSP47, in the presence or absence of eptifibatide, consistent with a role for HSP47 in enhancing platelet adhesion to collagen. Platelet adhesion under flow to von Willebrand factor was unaltered following HSP47 inhibition. Laser-induced thrombosis in cremaster muscle arterioles was reduced and bleeding time was prolonged in HSP47-deficient mice or following inhibition of HSP47. Conclusions Our study demonstrates the presence of HSP47 on the platelet surface, where it interacts with collagen, stabilizes platelet adhesion and increases collagen-mediated signalling and therefore thrombus formation and hemostasis.

Keywords: HSP47; chaperone; hemostasis; platelets; thrombosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism*
  • Calcium Signaling
  • Carrier Proteins / antagonists & inhibitors
  • Carrier Proteins / blood*
  • Carrier Proteins / genetics
  • Collagen / blood*
  • Disease Models, Animal
  • HSP70 Heat-Shock Proteins / antagonists & inhibitors
  • HSP70 Heat-Shock Proteins / blood*
  • HSP70 Heat-Shock Proteins / deficiency
  • HSP70 Heat-Shock Proteins / genetics
  • Hemostasis* / drug effects
  • Humans
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Platelet Activation* / drug effects
  • Platelet Adhesiveness
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Membrane Glycoproteins / metabolism
  • Protein Binding
  • Thrombosis / blood*
  • Thrombosis / genetics
  • Thrombosis / prevention & control

Substances

  • Carrier Proteins
  • HSP70 Heat-Shock Proteins
  • HSPA9 protein, human
  • Hspa9 protein, mouse
  • Platelet Aggregation Inhibitors
  • Platelet Membrane Glycoproteins
  • platelet membrane glycoprotein VI
  • Collagen