Peripheral tissues reprogram CD8+ T cells for pathogenicity during graft-versus-host disease

JCI Insight. 2018 Mar 8;3(5):e97011. doi: 10.1172/jci.insight.97011.


Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic stem cell transplantation induced by the influx of donor-derived effector T cells (TE) into peripheral tissues. Current treatment strategies rely on targeting systemic T cells; however, the precise location and nature of instructions that program TE to become pathogenic and trigger injury are unknown. We therefore used weighted gene coexpression network analysis to construct an unbiased spatial map of TE differentiation during the evolution of GVHD and identified wide variation in effector programs in mice and humans according to location. Idiosyncrasy of effector programming in affected organs did not result from variation in T cell receptor repertoire or the selection of optimally activated TE. Instead, TE were reprogrammed by tissue-autonomous mechanisms in target organs for site-specific proinflammatory functions that were highly divergent from those primed in lymph nodes. In the skin, we combined the correlation-based network with a module-based differential expression analysis and showed that Langerhans cells provided in situ instructions for a Notch-dependent T cell gene cluster critical for triggering local injury. Thus, the principal determinant of TE pathogenicity in GVHD is the final destination, highlighting the need for target organ-specific approaches to block immunopathology while avoiding global immune suppression.

Keywords: Immunology; Molecular pathology; Stem cell transplantation; T cell development; Transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism
  • Bone Marrow Transplantation / adverse effects
  • Cells, Cultured
  • Cellular Reprogramming / genetics
  • Cellular Reprogramming / immunology*
  • Disease Models, Animal
  • Female
  • Gene Expression Regulation / immunology
  • Graft vs Host Disease / immunology*
  • Graft vs Host Disease / pathology
  • Hematopoietic Stem Cell Transplantation / adverse effects
  • Humans
  • Langerhans Cells / immunology*
  • Langerhans Cells / metabolism
  • Lectins, C-Type / genetics
  • Lectins, C-Type / metabolism
  • Male
  • Mannose-Binding Lectins / genetics
  • Mannose-Binding Lectins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Multigene Family / genetics
  • Multigene Family / immunology
  • Primary Cell Culture
  • Receptors, Notch / metabolism
  • Skin / cytology
  • Skin / immunology*
  • Skin / pathology
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Transplantation Chimera
  • Transplantation, Homologous / adverse effects


  • Antigens, CD
  • Antigens, Surface
  • CD207 protein, human
  • Cd207 protein, mouse
  • Lectins, C-Type
  • Mannose-Binding Lectins
  • Receptors, Notch