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. 2018 Mar 18;39(2):58-71.
doi: 10.24272/j.issn.2095-8137.2017.012.

In vivo Genome Editing Thrives With Diversified CRISPR Technologies

Free PMC article

In vivo Genome Editing Thrives With Diversified CRISPR Technologies

Xun Ma et al. Zool Res. .
Free PMC article


Prokaryotic type II adaptive immune systems have been developed into the versatile CRISPR technology, which has been widely applied in site-specific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility. Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies. These new advances have significantly expanded the generation of genetically modified animal models, either by including species in which targeted genetic modification could not be achieved previously, or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods. Herein, we review the recent developments and applications of CRISPR-based technology in generating various animal models, and discuss the everlasting impact of this new progress on biomedical research.

Keywords: Animal models; CRISPR/Cas9; Genome editing.

Conflict of interest statement

The authors declare that they have no competing interests.

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Grant support

This study was supported by funds provided by the Research Grants Council of Hong Kong (CUHK 14104614, TBF16ENG007 and TBF17MED002 to B.F.; and 3132966 to W.Y.C.), and in part by funds from the Croucher Foundation (CAS16CU01/ CAS16401 to W.Y.C.) and the National Basic Research Program of China (973 Program, 2015CB964700 to Y.L.)