Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

doi:10.1128/genomeA.00097-18

. 2018 Mar 8;6(10):e00097-18.

doi: 10.1128/genomeA.00097-18.

Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

Jingchao Chen¹, Yi Li¹, Kun Zhang¹, Hailei Wang^{2

3}

Affiliations

¹ Singapore-China Joint Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, China.
² Singapore-China Joint Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, China whl@htu.cn.
³ Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore, Singapore.

PMID: 29519826
PMCID: PMC5843723
DOI: 10.1128/genomeA.00097-18

Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

Jingchao Chen et al. Genome Announc. 2018.

. 2018 Mar 8;6(10):e00097-18.

doi: 10.1128/genomeA.00097-18.

Authors

Jingchao Chen¹, Yi Li¹, Kun Zhang¹, Hailei Wang^{2

3}

Affiliations

¹ Singapore-China Joint Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, China.
² Singapore-China Joint Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, China whl@htu.cn.
³ Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore, Singapore.

PMID: 29519826
PMCID: PMC5843723
DOI: 10.1128/genomeA.00097-18

Abstract

The genomes of many strains of Escherichia coli have been sequenced, as this organism is a classic model bacterium. Here, we report the genome sequence of Escherichia coli DH5α, which is resistant to a T4 bacteriophage (CCTCC AB 2015375), while its other homologous E. coli strains, such as E. coli BL21, DH10B, and MG1655, are not resistant to phage invasions. Thus, understanding of the genome of the DH5α strain, along with comparative analysis of its genome sequence along with other sequences of E. coli strains, may help to reveal the bacteriophage resistance mechanism of E. coli.

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References

1. Singleton P. 1999. Bacteria in biology, biotechnology, and medicine 5th ed. Chichester, New York: John Wiley.
1. Chaudhuri RR, Henderson IR. 2012. The evolution of the Escherichia coli phylogeny. Infect Genet Evol 12:214–226. doi:10.1016/j.meegid.2012.01.005. - DOI - PubMed
1. Lee SY. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol 14:98–105. doi:10.1016/0167-7799(96)80930-9. - DOI - PubMed
1. Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS. 2011. PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352. doi:10.1093/nar/gkr485. - DOI - PMC - PubMed
1. Grissa I, Vergnaud G, Pourcel C. 2007. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res 35:W52–W57. doi:10.1093/nar/gkm360. - DOI - PMC - PubMed

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[1] Singleton P. 1999. Bacteria in biology, biotechnology, and medicine 5th ed. Chichester, New York: John Wiley.

[2] Singleton P. 1999. Bacteria in biology, biotechnology, and medicine 5th ed. Chichester, New York: John Wiley.

[3] Chaudhuri RR, Henderson IR. 2012. The evolution of the Escherichia coli phylogeny. Infect Genet Evol 12:214–226. doi:10.1016/j.meegid.2012.01.005. - DOI - PubMed

[4] Chaudhuri RR, Henderson IR. 2012. The evolution of the Escherichia coli phylogeny. Infect Genet Evol 12:214–226. doi:10.1016/j.meegid.2012.01.005. - DOI - PubMed

[5] Lee SY. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol 14:98–105. doi:10.1016/0167-7799(96)80930-9. - DOI - PubMed

[6] Lee SY. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol 14:98–105. doi:10.1016/0167-7799(96)80930-9. - DOI - PubMed

[7] Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS. 2011. PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352. doi:10.1093/nar/gkr485. - DOI - PMC - PubMed

[8] Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS. 2011. PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352. doi:10.1093/nar/gkr485. - DOI - PMC - PubMed

[9] Grissa I, Vergnaud G, Pourcel C. 2007. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res 35:W52–W57. doi:10.1093/nar/gkm360. - DOI - PMC - PubMed

[10] Grissa I, Vergnaud G, Pourcel C. 2007. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res 35:W52–W57. doi:10.1093/nar/gkm360. - DOI - PMC - PubMed

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Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

Affiliations

Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

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