Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33

Sof'ya N Senchenkova; Wenqi Hou; Olesya I Naumenko; Peng Geng; Alexander S Shashkov; Andrei V Perepelov; Bin Yang; Yuriy A Knirel

doi:10.1016/j.carres.2018.02.008

Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33

Carbohydr Res. 2018 May 2:460:47-50. doi: 10.1016/j.carres.2018.02.008. Epub 2018 Feb 17.

Authors

Sof'ya N Senchenkova¹, Wenqi Hou², Olesya I Naumenko³, Peng Geng⁴, Alexander S Shashkov¹, Andrei V Perepelov⁵, Bin Yang², Yuriy A Knirel¹

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
² Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, PR China.
³ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation; Higher Chemical College of the Russian Academy of Sciences, D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
⁴ School of Basic Medical Sciences, Tianjin Medical University, Heping District, Tianjin, 300070, PR China.
⁵ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: andreivperepelov@gmail.com.

PMID: 29524726
DOI: 10.1016/j.carres.2018.02.008

Abstract

An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D ¹H and ¹³C NMR spectroscopy: The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Glycerol phosphate; Lipopolysaccharide; O-antigen; O-antigen gene cluster; O-specific polysaccharide.

MeSH terms

Escherichia coli / chemistry*
Escherichia coli / genetics*
Lipopolysaccharides / chemistry*
Multigene Family / genetics
O Antigens / chemistry*
Polysaccharides, Bacterial / chemistry*

Substances

Lipopolysaccharides
O Antigens
Polysaccharides, Bacterial