Purification, characterization and immunomodulatory activity of polysaccharides from stem lettuce

Chenzhipeng Nie; Peilei Zhu; Shuping Ma; Mingchun Wang; Youdong Hu

doi:10.1016/j.carbpol.2018.02.009

Purification, characterization and immunomodulatory activity of polysaccharides from stem lettuce

Carbohydr Polym. 2018 May 15:188:236-242. doi: 10.1016/j.carbpol.2018.02.009. Epub 2018 Feb 7.

Authors

Chenzhipeng Nie¹, Peilei Zhu², Shuping Ma¹, Mingchun Wang³, Youdong Hu⁴

Affiliations

¹ Anhui Province Engineering Laboratory of Agricultural Products Processing, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
² Anhui Province Engineering Laboratory of Agricultural Products Processing, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China; Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
³ Anhui Province Engineering Laboratory of Agricultural Products Processing, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China. Electronic address: wmc@ahau.edu.cn.
⁴ Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, China.

PMID: 29525161
DOI: 10.1016/j.carbpol.2018.02.009

Abstract

Stem lettuce has a long history of cultivation in China and possesses high nutritional and medicinal value. In our previous studies, extraction optimization, characterization, and bioactivities of stem lettuce polysaccharides (SLP) were investigated. In this study, SLP were further separated into two purified polysaccharides, SLP-1 and SLP-2, by anion exchange chromatography followed by size exclusion chromatography. SLP-1, with a molecular weight of 90 KDa, was mainly composed of galacturonic acid, galactose and arabinose in a molar ratio of 17.6:41.7:33.9. SLP-2, with a molecular weight of 44 KDa, was mainly composed of mannose, galacturonic acid, galactose and arabinose in a molar ratio of 11.5:69.5:9.3:8.2. In addition, both purified polysaccharides contain sulphate radicals, have triple helical structures and can promote macrophage proliferation without cytotoxicity. SLP-2 was better able to stimulate phagocytic and nitric oxide production than SLP-1. The results suggest that polysaccharides from stem lettuce could be explored as immunomodulatory agents in the field of pharmaceuticals and functional foods.

Keywords: Characterization; Immunomodulatory; Polysaccharide; Purification; Stem lettuce.

MeSH terms

Animals
Arabinose / chemistry
Cell Proliferation / drug effects
Chromatography, Gel
Galactose / chemistry
Hexuronic Acids / chemistry
Immunologic Factors / chemistry*
Immunologic Factors / pharmacology
Lactuca / chemistry*
Macrophages / drug effects
Mice
Polysaccharides / chemistry*
Polysaccharides / pharmacology

Substances

Hexuronic Acids
Immunologic Factors
Polysaccharides
galacturonic acid
Arabinose
Galactose