The combination between cations and sulfated polysaccharide from abalone gonad (Haliotis discus hannai Ino)

Yiming Guo; Shuang Cong; Jun Zhao; Yingying Dong; Tingting Li; Beiwei Zhu; Shuang Song; Chengrong Wen

doi:10.1016/j.carbpol.2018.01.100

The combination between cations and sulfated polysaccharide from abalone gonad (Haliotis discus hannai Ino)

Carbohydr Polym. 2018 May 15:188:54-59. doi: 10.1016/j.carbpol.2018.01.100. Epub 2018 Feb 2.

Authors

Yiming Guo¹, Shuang Cong¹, Jun Zhao², Yingying Dong¹, Tingting Li¹, Beiwei Zhu¹, Shuang Song³, Chengrong Wen⁴

Affiliations

¹ School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.
² National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China; School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China.
³ School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China. Electronic address: songs1008@163.com.
⁴ School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China. Electronic address: wencr2014@163.com.

PMID: 29525171
DOI: 10.1016/j.carbpol.2018.01.100

Abstract

Effects of cations (Na⁺, K⁺, Ca²⁺, Mg²⁺) on the structure of abalone gonad sulfated polysaccharide (AGSP) were studied by means of Congo red test, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The results showed that the local ordered helical conformation was observed in AGSP. The cations could combine with anionic groups in AGSP by ionic bonds, which caused the weakening of electrostatic repulsion and crosslinking of molecular chains. Furthermore, the effects of divalent cations on the conformation were more obvious than that of monovalent cations, and divalent cations led to the aggregation of AGSP due to the more interaction sites. In short, AGSP molecular chains were crosslinked through ionic bonds after adding cations, and, divalent cations could induce the aggregation of AGSP by electrostatic interactions. This study will provide valuable insights for the further research on AGSP conformation.

Keywords: Abalone gonad sulfated polysaccharide; Cations; Conformation; Molecular interaction.

MeSH terms

Animals
Cations / chemistry*
Gastropoda / chemistry
Microscopy, Electron, Scanning
Polysaccharides / chemistry*
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Cations
Polysaccharides