Transfusions enriched for W3/25+ helper/inducer T lymphocytes prevent spontaneous diabetes in the BB/W rat

Diabetologia. 1987 Jan;30(1):22-6. doi: 10.1007/BF01788902.

Abstract

Transfusions of spleen cells are known to prevent spontaneous autoimmune diabetes in susceptible BB/W rats, while T cell-depleted transfusions are ineffective. To characterize further the protective cell(s), we transfused young diabetes prone rats with splenocytes from diabetes resistant BB/W rats that were treated in vitro to enrich them in either OX8+ (suppressor/cytotoxic) T cells or W3/25+ (helper/inducer) T cells. Diabetes subsequently occurred in 19 of 29 (66%) recipients of OX8-enriched, W3/25-depleted cells and 20 of 37 (54%) controls, but in only 7 of 30 (23%) recipients of W3/25-enriched, OX8-depleted cells (p less than 0.005). Transfusion of spleen cells from diabetes resistant donor rats pretreated in vivo to deplete OX8+ cells also prevented diabetes in susceptible BB/W recipients. We conclude that transfusions of W3/25+ helper/inducer splenic T lymphocytes obtained from diabetes resistant animals prevent spontaneous diabetes in the BB/W rat.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Diabetes Mellitus / immunology*
  • Diabetes Mellitus / physiopathology
  • Diabetes Mellitus / prevention & control
  • Female
  • Male
  • Rats
  • Rats, Inbred BB
  • Spleen / cytology
  • T-Lymphocytes, Cytotoxic / physiology*
  • T-Lymphocytes, Helper-Inducer / physiology*
  • T-Lymphocytes, Regulatory / physiology*

Substances

  • Antibodies, Monoclonal