Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

Methods Mol Biol. 2018;1761:209-220. doi: 10.1007/978-1-4939-7747-5_16.

Abstract

Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions.

Keywords: Arabidopsis thaliana; Cross-linked beads; Cytosolic fraction; Immunoprecipitation; Integral membrane proteins; Membrane fraction; Peripheral membrane proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / isolation & purification
  • Arabidopsis Proteins / metabolism*
  • Blotting, Western
  • Cell Membrane / metabolism
  • Immunoprecipitation* / methods
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Plant Roots / metabolism*

Substances

  • Arabidopsis Proteins
  • Membrane Proteins