Reinforced chitosan membranes by microspheres for guided bone regeneration

Di Huang; Lulu Niu; Jian Li; Jingjing Du; Yan Wei; Yinchun Hu; Xiaojie Lian; Weiyi Chen; Kaiqun Wang

doi:10.1016/j.jmbbm.2018.03.006

Reinforced chitosan membranes by microspheres for guided bone regeneration

J Mech Behav Biomed Mater. 2018 May:81:195-201. doi: 10.1016/j.jmbbm.2018.03.006. Epub 2018 Mar 7.

Authors

Di Huang¹, Lulu Niu², Jian Li³, Jingjing Du⁴, Yan Wei³, Yinchun Hu³, Xiaojie Lian³, Weiyi Chen⁵, Kaiqun Wang⁶

Affiliations

¹ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, PR China; Institute of Applied Mechanics & Biomedical Engineering, Shanxi Key Laboratory of Material Strength & Structural Impact, Taiyuan University of Technology, Taiyuan 030024, PR China.
² Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, PR China; Research Center for Nano-Biomaterials, Analytical & Testing Center, Sichuan University, Chengdu 610064, PR China.
³ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, PR China.
⁴ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, PR China. Electronic address: dujingjing198539@163.com.
⁵ Institute of Applied Mechanics & Biomedical Engineering, Shanxi Key Laboratory of Material Strength & Structural Impact, Taiyuan University of Technology, Taiyuan 030024, PR China.
⁶ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, PR China. Electronic address: wangkaiqun@tyut.edu.cn.

PMID: 29529590
DOI: 10.1016/j.jmbbm.2018.03.006

Abstract

In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane. The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine. The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix. The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres. Its elongation rate at break reaches 5.61 ± 0.95%. The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively. Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m², which is about 3 times of the pure CS membrane. The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.

Keywords: Chitosan; Cytocompatibility; Guided bone regeneration; Nano-hydroxyapatite/chitosan microspheres; Reinforce.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Regeneration / drug effects*
Cell Line
Cell Proliferation / drug effects
Chitosan / chemistry*
Chitosan / pharmacology*
Guided Tissue Regeneration / methods*
Materials Testing
Mechanical Phenomena*
Membranes, Artificial*
Microspheres*
Osteoblasts / cytology
Osteoblasts / drug effects

Substances

Membranes, Artificial
Chitosan