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. 2018 Apr 26;62(5):e02445-17.
doi: 10.1128/AAC.02445-17. Print 2018 May.

Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas Aeruginosa

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Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas Aeruginosa

Paulo Juarez et al. Antimicrob Agents Chemother. .
Free PMC article

Abstract

When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of Pseudomonas aeruginosa to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene mexT result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates.

Keywords: MexEF-OprN; MexT; Pseudomonas aeruginosa; antibiotic resistance.

Figures

FIG 1
FIG 1
Crystal structure of DntR dimer from Burkholderia spp. (PDB 5AE5) (first reported in reference 15). One monomer is colored to indicate the functions of its constituting domains (green for the coinducer binding domain, blue for the DNA binding domain, and orange for the loop linking the two domains). The second monomer is in yellow. Phenylalanine-257 residues of the two monomers are highlighted in red.

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