Transplantation of in vitro expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine's cornea has been earlier reported. Herein we report the transplantation of HCEP cells derived from a cadaver donor cornea to three patients using the NC gel sheet. In three patients with bullous keratopathy, one after cataract surgery, one after trauma and another in the corneal graft, earlier performed for congenital corneal dystrophy, not amenable to medical management HCEP cells isolated from a human cadaver donor cornea in vitro expanded using a thermoreversible gelation polymer (TGP) for 26 days were divided into three equal portions and 1.6 × 105 HCEP cells were injected on to the endothelium of the affected eye in each patient using the D25-NC gel sheet as a supporting material. The sheets were removed after three days. The bullae in the cornea disappeared by the 3rd-11th post-operative day in all the three patients. Visual acuity improved from Perception of light (PL)+/Projection of rays (PR)+ to Hand movements (HM)+ in one of the patients by post-operative day 3 which was maintained at 18 months follow-up. At 18 months follow-up, in another patient the visual acuity had improved from HM+ to 6/60 while in the third patient, visual acuity remained HM+ as it was prior to HCEP transplantation. There were no adverse effects during the follow-up in any of the patients.
Keywords: Corneal dystrophy; cell transplantation; hydrogel; nanocomposites.
Conflict of interest statement
Authors Abraham and Haraguchi are applicants to a patent on usage of NC gel sheet. Author Parikumar is a stake holder in the Light eye hospital, Dharmapuri, India.
Successful Transplantation of in Vitro Expanded Human Cadaver Corneal Endothelial Precursor Cells on to a Cadaver Bovine's Eye Using a Nanocomposite Gel SheetP Parikumar et al. Curr Eye Res 39 (5), 522-6. PMID 24144454.The use of the NC gel sheet makes HCEP cell transplantation feasible for human patients. Further in vitro basic studies followed by translational studies are necessary to …
Successful Transplantation of in Vitro Expanded Human Corneal Endothelial Precursors to Corneal Endothelial Surface Using a Nanocomposite SheetsP Parikumar et al. J Stem Cells Regen Med 7 (2), 94. PMID 24693181.Transplantation of in vitro expanded donor human corneal endothelial cells using a transparent Nano-composite sheet was feasible in bovine eyes and the HCEC an engrafted …
[Cultured Human Corneal Endothelial Cell Transplantation]T Mimura. Nippon Ganka Gakkai Zasshi 110 (11), 879-97. PMID 17134036. - ReviewResearchers have demonstrated the feasibility of transplanting human cultured corneal endothelial cells (HCEC) in various animal models. This review provides an overview …
Complex Deep Lamellar Endothelial Keratoplasty for Complex Bullous Keratopathy With Severe Vision LossT Huang et al. Cornea 28 (2), 157-62. PMID 19158557.In cases of complex bullous keratopathy with severe vision loss, DLEK combined with other intraocular surgeries is a feasible and effective procedure to significantly imp …
[Cultivated Corneal Endothelial Cell Sheet Transplantation in a Primate Model]N Koizumi. Nippon Ganka Gakkai Zasshi 113 (11), 1050-9. PMID 19994583. - ReviewOur new surgical treatment for corneal endothelial dysfunction involves replacing damaged the corneal endothelium with healthy corneal endothelial cells cultivated and mu …
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