Arabidopsis TSO1 and MYB3R1 form a regulatory module to coordinate cell proliferation with differentiation in shoot and root

Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):E3045-E3054. doi: 10.1073/pnas.1715903115. Epub 2018 Mar 13.


Fundamental to plant and animal development is the regulated balance between cell proliferation and differentiation, a process intimately tied to cell cycle regulation. In Arabidopsis, mutations in TSO1, whose animal homolog is LIN54, resulted in severe developmental abnormalities both in shoot and root, including shoot meristem fasciation and reduced root meristematic zone. The molecular mechanism that could explain the tso1 mutant phenotype is absent. Through a genetic screen, we identified 32 suppressors that map to the MYB3R1 gene, encoding a conserved cell cycle regulator. Further analysis indicates that TSO1 transcriptionally represses MYB3R1, and the ectopic MYB3R1 activity mediates the tso1 mutant phenotype. Since animal homologs of TSO1 and MYB3R1 are components of a cell cycle regulatory complex, the DREAM complex, we tested and showed that TSO1 and MYB3R1 coimmunoprecipitated in tobacco leaf cells. Our work reveals a conserved cell cycle regulatory module, consisting of TSO1 and MYB3R1, for proper plant development.

Keywords: DREAM complex; MYB3R1; TSO1; cell cycle; meristem.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Differentiation*
  • Cell Proliferation*
  • DNA, Plant
  • Gene Expression Regulation, Plant*
  • Phenotype
  • Plant Roots / cytology*
  • Plant Roots / metabolism
  • Plant Shoots / cytology*
  • Plant Shoots / metabolism
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*


  • Arabidopsis Proteins
  • DNA, Plant
  • MYB3R1 protein, Arabidopsis
  • TSO1 protein, Arabidopsis
  • Trans-Activators