Interaction of Antibiotics With Functional Sites in 16S Ribosomal RNA

Nature. 1987 Jun 4-10;327(6121):389-94. doi: 10.1038/327389a0.

Abstract

Chemical footprinting shows that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin and the neomycins) protect concise sets of highly conserved nucleotides in 16S ribosomal RNA when bound to ribosomes. These findings have strong implications for the mechanism of action of these antibiotics and for the assignment of functions to specific structural features of 16S rRNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoglycosides / pharmacology
  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Cinnamates*
  • Edeine / pharmacology
  • Hygromycin B / analogs & derivatives
  • Hygromycin B / pharmacology
  • Nucleic Acid Conformation / drug effects
  • RNA, Ribosomal / drug effects
  • RNA, Ribosomal / genetics*
  • Spectinomycin / pharmacology
  • Streptomycin / pharmacology
  • Tetracycline / pharmacology

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Cinnamates
  • RNA, Ribosomal
  • Edeine
  • Hygromycin B
  • hygromycin A
  • Spectinomycin
  • Tetracycline
  • Streptomycin