Household light source for potent photo-dynamic antimicrobial effect and wound healing in an infective animal model

Azeem Ullah; Yuxiang Zhang; Zafar Iqbal; Yaxin Zhang; Dong Wang; Jincan Chen; Ping Hu; Zhuo Chen; Mingdong Huang

doi:10.1364/BOE.9.001006

Household light source for potent photo-dynamic antimicrobial effect and wound healing in an infective animal model

Biomed Opt Express. 2018 Feb 6;9(3):1006-1019. doi: 10.1364/BOE.9.001006. eCollection 2018 Mar 1.

Authors

Azeem Ullah^{1

2

3}, Yuxiang Zhang^{1

2

3}, Zafar Iqbal^{1

4}, Yaxin Zhang^{1

2}, Dong Wang^{1

2}, Jincan Chen¹, Ping Hu¹, Zhuo Chen^{1

2

5}, Mingdong Huang^{1

6

7}

Affiliations

¹ State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, 155 West Yangqiao Road, Fuzhou, Fujian, China, 350002.
² University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Shijingshan District, Beijing, China, 100049.
³ The first two authors contributed equally to this work.
⁴ Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan, 22060.
⁵ zchen@fjirsm.ac.cn.
⁶ Fuzhou University, Fujian, China, 350002.
⁷ mhuang@fjirsm.ac.cn.

Abstract

Photodynamic antimicrobial chemotherapy (PACT) is considered a promising alternative to conventional antibiotic approach. We have previously developed a novel PS containing five lysine amino acids, pentalysine-β-carbonylphthalocyanine Zinc (ZnPc(Lys)₅), which in the presence of light, is highly toxic against a range of bacterial strains, including hospital isolated, drug resistant Acinetobacter baumannii. Here, we study the effect of light fluence of the two light sources on the PACT potency of ZnPc(Lys)₅. We observed that an exposure of E.coli to a red LED light for only 2 seconds (light fluence of 0.15 J/cm²) in the presence of ZnPc(Lys)₅ significantly eradicated 80% of the E.coli. We further demonstrated that a light fluence of 4.5 J/cm² from a household light source induced a noticeable photodynamic effect in vitro and in vivo animal model. This study points to a new research direction of reducing light illumination time by increasing potency of PS.

Keywords: (170.1870) Dermatology; (170.3890) Medical optics instrumentation; (170.5180) Photodynamic therapy; (230.3670) Light-emitting diodes.