The primary structure of the beta-subunit of the cell surface adhesion glycoproteins LFA-1, CR3 and p150,95 and its relationship to the fibronectin receptor

EMBO J. 1987 Apr;6(4):915-9.

Abstract

The lymphocyte-function-associated antigen-1 (LFA-1), the complement receptor type 3 (CR3) and the antigen p150,95 are cell-surface glycoproteins. They are heterodimeric complexes, each containing a unique alpha-subunit noncovalently associated with a common beta-subunit. We have purified the beta-subunit from human spleen and obtained limited peptide sequences. What appears to be the complete primary structure for the fully processed beta-subunit was obtained by cDNA sequencing of clones from a phorbol ester (PMA) stimulated U937 cDNA library. There are five possible glycosylation sites and a transmembrane segment. The sequence contains a high level of cysteine (7.6%), with 24 of the 57 cysteine residues being found in three repeating units each with eight residues. The entire primary structure has 47% identity to a subunit of a fibronectin binding protein from chicken fibroblasts. It seems that LFA-1, CR3 and p150,95 antigens may belong to an extended family of cell surface molecules including the fibronectin binding protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Surface / genetics*
  • Antigens, Surface / isolation & purification
  • Base Sequence
  • Cloning, Molecular
  • DNA / analysis
  • Fibronectins / metabolism
  • Genes
  • Humans
  • Lymphocyte Function-Associated Antigen-1
  • Macromolecular Substances
  • Macrophage-1 Antigen
  • Receptors, Complement / genetics*
  • Receptors, Fibronectin
  • Receptors, Immunologic / genetics*
  • Spleen / immunology
  • Spleen / metabolism

Substances

  • Antigens, Surface
  • Fibronectins
  • Lymphocyte Function-Associated Antigen-1
  • Macromolecular Substances
  • Macrophage-1 Antigen
  • Receptors, Complement
  • Receptors, Fibronectin
  • Receptors, Immunologic
  • DNA

Associated data

  • GENBANK/M19545
  • GENBANK/Y00057