Determination of the antimicrobial susceptibility and molecular profile of clarithromycin resistance in the Mycobacterium abscessus complex in Japan by variable number tandem repeat analysis

Mari Kusuki; Kayo Osawa; Kentaro Arikawa; Miho Tamura; Katsumi Shigemura; Toshiro Shirakawa; Tatsuya Nakamura; Yuji Nakamachi; Masato Fujisawa; Jun Saegusa; Issei Tokimatsu

doi:10.1016/j.diagmicrobio.2018.02.008

Determination of the antimicrobial susceptibility and molecular profile of clarithromycin resistance in the Mycobacterium abscessus complex in Japan by variable number tandem repeat analysis

Diagn Microbiol Infect Dis. 2018 Jul;91(3):256-259. doi: 10.1016/j.diagmicrobio.2018.02.008. Epub 2018 Feb 18.

Authors

Affiliations

¹ Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan; Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan.
² Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan; Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan. Electronic address: osawak@kobe-u.ac.jp.
³ Department of Infectious Diseases, Kobe Institute of Health, Kobe, Japan.
⁴ Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
⁵ Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan; Department of International Health, Kobe University Graduate School of Health Sciences, Kobe, Japan; Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
⁶ Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan; Division of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Kobe, Japan.
⁷ Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan.
⁸ Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
⁹ Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan.

PMID: 29550059
DOI: 10.1016/j.diagmicrobio.2018.02.008

Abstract

Mycobacterium abscessus complex, including three subspecies-M. abscessus, M. massiliense, and M. bolletii-is resistant to a variety of antibiotics so limited treatment options are available. The susceptibility of these subspecies to antimicrobial agents depends in particular on the erm(41) sequevar and rrl mutations in the 23S rRNA, which are potentially related to clarithromycin (CLR) resistance. The purpose of this study was to carry out identification and molecular characterization of these subspecies based on variable number of tandem repeats (VNTR) analysis. Twenty-four M. abscessus complex strains were identified as M. abscessus and M. massiliense and these subspecies could be discriminated between based on their resistance to CLR, as determined by truncation or mutation of erm(41) or mutation of rrl, as illustrated by their VNTR patterns. In conclusion, we confirmed that the CLR susceptibility profiles could be differentiated according to the subspecies of M. abscessus complex strains by their VNTR patterns.

Keywords: Mycobacterium abscessus complex; VNTR analysis; erm (41); rrl.

MeSH terms

Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents / pharmacology*
Clarithromycin / pharmacology*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Drug Resistance, Bacterial*
Female
Humans
Japan
Male
Microbial Sensitivity Tests / methods*
Middle Aged
Minisatellite Repeats*
Molecular Diagnostic Techniques / methods
Mycobacterium Infections, Nontuberculous / microbiology
Mycobacterium abscessus / drug effects*
Mycobacterium abscessus / genetics*
RNA, Ribosomal, 23S / genetics

Substances

Anti-Bacterial Agents
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 23S
Clarithromycin