A surface magnetic imprinted polymers as artificial receptors for selective and efficient capturing of new neuronal nitric oxide synthase-post synaptic density protein-95 uncouplers

J Pharm Biomed Anal. 2018 May 30:154:180-190. doi: 10.1016/j.jpba.2018.03.003. Epub 2018 Mar 3.


In this work, surface magnetic molecularly imprinted polymers (SMMIPs) were synthesized and used as artificial receptors in the dispersive magnetic solid phase extraction (DMSPE) for capturing potential neuronal nitric oxide synthase-post synaptic density protein-95 (nNOS-PSD-95) uncouplers, which is known as neuroprotection against stroke. Factors that affected selective separation and adsorption of the artificial receptors, such as the amount of template, the types of functional monomer and porogen solvents, and the molar ratio of template/functional monomer/cross-linker were optimized. The artificial receptors were also characterized using fourier transformed infrared, scanning electron microscope, thermal gravimetric analysis and physical property measurement systems. Multiple interactions between template and SMMIPs led to larger binding capacities, faster binding kinetics, quicker separation abilities and more efficient selectivity than the surface magnetic nonimprinted polymers (SMNIPs). The SMMIPs were successfully applied to capture potential nNOS-PSD-95 uncouplers from complex samples, and eight compounds were seized and confirmed rapidly when combined with HPLC and MS. The detection of the new nNOS-PSD-95 uncouplers ranged from 0.001 to 1.500 mg/mL with correlation coefficients of 0.9990-0.9995. The LOD and LOQ were 0.10-0.68 μg/mL and 0.47-2.11 μg/mL, respectively. The neuroprotective effect and co-immunoprecipitation test in vitro revealed that Emodin-1-O-β-d-glucoside, Rhaponticin, Gnetol and 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucoside have neuroprotective and uncoupling activities, and that they may be the new uncouplers of nNOS-PSD-95.

Keywords: Artificial receptors; Dispersive solid phase extraction; Magnetic molecularly imprinted polymers; Neuronal nitric oxide synthase–post synaptic density protein-95 uncouplers; Stroke.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Disks Large Homolog 4 Protein / chemistry*
  • Emodin / chemistry
  • Glucosides / chemistry
  • Magnetics / methods
  • Molecular Imprinting / methods
  • Neuroprotective Agents / chemistry
  • Nitric Oxide Synthase Type I / chemistry*
  • PC12 Cells
  • Polymers / chemistry*
  • Post-Synaptic Density / chemistry*
  • Rats
  • Receptors, Artificial / chemistry*
  • Solid Phase Extraction / methods
  • Solvents / chemistry
  • Stilbenes / chemistry


  • 2,3',5',6-tetrahydroxy-trans-stilbene
  • Disks Large Homolog 4 Protein
  • Glucosides
  • Neuroprotective Agents
  • Polymers
  • Receptors, Artificial
  • Solvents
  • Stilbenes
  • 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside
  • Nitric Oxide Synthase Type I
  • rhapontin
  • Emodin