Effects of the cross-linkers on the buckling of microtubules in cells

Si Li; Chengyuan Wang; Perumal Nithiarasu

doi:10.1016/j.jbiomech.2018.03.002

Effects of the cross-linkers on the buckling of microtubules in cells

J Biomech. 2018 Apr 27:72:167-172. doi: 10.1016/j.jbiomech.2018.03.002. Epub 2018 Mar 12.

Authors

Si Li¹, Chengyuan Wang², Perumal Nithiarasu¹

Affiliations

¹ Zienkiewicz Centre for Computational Engineering, College of Engineering, Swansea University, Bay Campus, Fabian Way, Swansea, Wales SA1 8EN, UK.
² Zienkiewicz Centre for Computational Engineering, College of Engineering, Swansea University, Bay Campus, Fabian Way, Swansea, Wales SA1 8EN, UK. Electronic address: chengyuan.wang@swansea.ac.uk.

PMID: 29551426
DOI: 10.1016/j.jbiomech.2018.03.002

Abstract

In cells, the protein cross-linkers lead to a distinct buckling behavior of microtubules (MTs) different from the buckling of individual MTs. This paper thus aims to examine this issue via the molecular structural mechanics (MSM) simulations. The transition of buckling responses was captured as the two-dimensional-linkers were replaced by the three-dimensional (3D) ones. Then, the effects of the radial orientation and the axial density of the 3D-linkers were examined, showing that more uniform distribution of the radial orientation leads to the higher critical load with 3D buckling modes, while the inhomogeneity of the axial density results in the localized buckling patterns. The results demonstrated the important role of the cross-linker in regulating MT stiffness, revealed the physics of the experimentally observed localized buckling and these results will pave the way to a new multi-component mechanics model for whole cells.

Keywords: 3D localized buckling; Local density of linkers; Microtubules; Protein cross-linkers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Microtubules / physiology*
Models, Molecular*
Proteins / physiology*

Substances

Proteins