In vivo high resolution human corneal imaging using full-field optical coherence tomography

Viacheslav Mazlin; Peng Xiao; Eugénie Dalimier; Kate Grieve; Kristina Irsch; José-Alain Sahel; Mathias Fink; A Claude Boccara

doi:10.1364/BOE.9.000557

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Biomed Opt Express. 2018 Jan 10;9(2):557-568. doi: 10.1364/BOE.9.000557. eCollection 2018 Feb 1.

Authors

Viacheslav Mazlin¹, Peng Xiao¹, Eugénie Dalimier², Kate Grieve^{3

4}, Kristina Irsch^{3

4

5}, José-Alain Sahel^{3

4

6}, Mathias Fink¹, A Claude Boccara^{1

2}

Affiliations

¹ Institute Langevin, ESPCI PARIS, PSL Research University, 1 Rue Jussieu, Paris, 75005, France.
² LLTech SAS, 29 Rue du Faubourg Saint Jacques, Paris, 75014, France.
³ Vision Institute/CIC 1423, UPMC-Sorbonne Universities, UMR_S 968/INSERM, U968/CNRS, UMR_7210, 17 Rue Moreau, Paris, 75012, France.
⁴ Quinze-Vingts National Eye Hospital, 28 Rue de Charenton, Paris, 75012, France.
⁵ Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, 600 N Wolfe Street, Baltimore, MD 21287, USA.
⁶ Department of Ophthalmology, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15213, USA.

Abstract

We present the first full-field optical coherence tomography (FFOCT) device capable of in vivo imaging of the human cornea. We obtained images of the epithelial structures, Bowman's layer, sub-basal nerve plexus (SNP), anterior and posterior stromal keratocytes, stromal nerves, Descemet's membrane and endothelial cells with visible nuclei. Images were acquired with a high lateral resolution of 1.7 µm and relatively large field-of-view of 1.26 mm x 1.26 mm - a combination, which, to the best of our knowledge, has not been possible with other in vivo human eye imaging methods. The latter together with a contactless operation, make FFOCT a promising candidate for becoming a new tool in ophthalmic diagnostics.

Keywords: (110.4500) Optical coherence tomography; (170.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging; (170.3890) Medical optics instrumentation; (170.4460) Ophthalmic optics and devices.