Inactivation of enteroviruses by ascorbic acid and sodium bisulfite

Appl Environ Microbiol. 1978 Jul;36(1):68-75. doi: 10.1128/aem.36.1.68-75.1978.


Poliovirus type 1, coxsackievirus type A9, and echovirus type 7 were inactivated by sodium bisulfite and ascorbic acid. Inactivation rates depended upon concentration, temperature, and pH. RNA infectivity was lost during inactivation; the capsid was also altered by these inactivating agents, as determined by enzyme sensitivity assays and by tests of adsorption to cells. Structural modifications of the virus particles were not identical, suggesting that the mechanism of inactivation by ascorbic acid differs from that of sodium bisulfite.

Publication types

  • Comparative Study

MeSH terms

  • Ascorbic Acid / pharmacology*
  • Echovirus 9 / drug effects*
  • Echovirus 9 / metabolism
  • Enterovirus / drug effects*
  • Enterovirus / metabolism
  • Enterovirus B, Human / drug effects*
  • Hemagglutinins, Viral
  • Hydrogen-Ion Concentration
  • Poliovirus / drug effects*
  • Poliovirus / metabolism
  • RNA, Viral / metabolism
  • Sulfites / pharmacology*
  • Temperature


  • Hemagglutinins, Viral
  • RNA, Viral
  • Sulfites
  • Ascorbic Acid