Deregulated expression of HDAC3 in colorectal cancer and its clinical significance

Adv Clin Exp Med. 2018 Mar;27(3):305-311. doi: 10.17219/acem/66207.

Abstract

Background: To date, 4 classes of histone deacetylases (HDACs) have been identified in humans. Class I HDACs are zinc-dependent and NAD+-independent enzymes, and include 4 isoforms closely related to yeast RPD3: HDAC1, 2, 3, and 8.

Objectives: The aims of the study were to quantitatively evaluate the expression of HDAC3 in colorectal cancer (CRC) and to correlate its expression levels with clinicopathological parameters.

Material and methods: We characterized expression patterns of HDAC3 as class I HDAC isoforms in a cohort of 48 CRC patients by quantitative (real-time) reverse transcription polymerase chain reaction (RT-PCR). In addition, the potential relationship between HDAC3 expression levels and clinicopathological parameters in patients suffering from CRC was explored.

Results: We found that HDAC3 was highly expressed in colorectal tumors compared to normal colorectal tissues (p < 0.05). Furthermore, we found significant correlations between HDAC3 expression levels and tumor differentiation grades (p < 0.05).

Conclusions: In this prospective study we identified a pronounced HDAC3 expression pattern in CRC. Our findings support an important role of HDAC3 as a complementary molecular marker for existing histopathological diagnostic elements; it might also have applications in prognostic and targeted therapy. Furthermore, HDAC3 can be used as a biomarker to differentiate between tumor borders and margins, and it may also be useful for characterizing field cancerization in CRC.

Keywords: HDAC3; colorectal cancer; deregulation; prognosis.

MeSH terms

  • Cell Proliferation
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology
  • Colorectal Neoplasms / enzymology*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology
  • Disease Progression
  • Down-Regulation
  • Histone Deacetylases / genetics
  • Histone Deacetylases / metabolism*
  • Humans
  • Prognosis
  • Prospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Histone Deacetylases