Synthesis of Some Novel Thiadiazole Derivative Compounds and Screening Their Antidepressant-Like Activities

Molecules. 2018 Mar 21;23(4):716. doi: 10.3390/molecules23040716.

Abstract

Novel thiadiazole derivatives were synthesized through the reaction of acetylated 2-aminothiadiazole and piperazine derivatives. The chemical structures of the compounds were clarified by Infrared Spectroscopy (IR), ¹H Nuclear Magnetic Resonance Spectroscopy (¹H-NMR), 13C Nuclear Magnetic Resonance Spectroscopy (13C-NMR) and Electronspray Ionisation Mass Spectroscopy (ESI-MS) spectroscopic methods. Antidepressant-like activities were evaluated by the tail-suspension (TST) and modified forced swimming (MFST) methods. Besides, possible influence of the test compounds on motor activities of the animals were examined by activity cage tests. In the TST, administration of the compounds 2c, 2d, 2e, 2f, 2g and 2h significantly decreased the immobility time of mice regarding the control values. Further, in the MFST, the same compounds reduced the total number of immobility behaviors while increasing swimming performance. However, no change was observed in the total number of climbing behaviors. These data suggested that compounds 2c, 2d, 2e, 2f, 2g and 2h possess notable antidepressant-like activities. Reference drug fluoxetine (10 mg/kg) was also exhibited its antidepressant activity, as expected. No significant difference was seen between the locomotor activity values of the test groups signifying that observed antidepressant-like activities are specific. Theoretical calculation of absorption, distribution, metabolism, excretion (ADME) properties for the obtained compounds were performed and obtained data supported the antidepressant-like potential of these novel thiadiazole derivatives.

Keywords: activity cage; modified forced swimming; tail-suspension; thiadiazole.

MeSH terms

  • Animals
  • Antidepressive Agents / chemical synthesis*
  • Antidepressive Agents / metabolism
  • Antidepressive Agents / pharmacology*
  • Drug Evaluation, Preclinical*
  • Fluoxetine / chemical synthesis
  • Fluoxetine / pharmacology
  • Hindlimb Suspension
  • Male
  • Mice, Inbred BALB C
  • Motor Activity / drug effects
  • Swimming
  • Thiadiazoles / chemical synthesis*
  • Thiadiazoles / metabolism
  • Thiadiazoles / pharmacology*

Substances

  • Antidepressive Agents
  • Thiadiazoles
  • Fluoxetine