Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models

Methods Mol Biol. 2018;1753:103-113. doi: 10.1007/978-1-4939-7720-8_7.


A monolayer of pigmented epithelial cells, the retinal pigment epithelium (RPE), supports photoreceptor function in many ways. Consistent with these roles, RPE dysfunction underlies a number of hereditary retinal disorders. To monitor RPE function in vivo models for these conditions, we adapted an electroretinographic (ERG) technique based on direct current amplification (DC-ERG). This chapter describes the main features of this approach and its application to mouse models involving the RPE.

Keywords: C-wave; Direct current electroretinography; Fast oscillation; Light peak; Off-response; Retinal pigment epithelium.

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Electrodes
  • Electroretinography / instrumentation
  • Electroretinography / methods*
  • Mice
  • Photic Stimulation / instrumentation
  • Photic Stimulation / methods
  • Retinal Diseases / diagnostic imaging*
  • Retinal Diseases / pathology
  • Retinal Pigment Epithelium / diagnostic imaging*
  • Retinal Pigment Epithelium / pathology