Mulberry Leaf Extracts prevent obesity-induced NAFLD with regulating adipocytokines, inflammation and oxidative stress

J Food Drug Anal. 2018 Apr;26(2):778-787. doi: 10.1016/j.jfda.2017.10.008. Epub 2017 Dec 2.


Mulberry (Morus alba) leaf has been used in Chinese medicine as the remedy for hyperlipidemia and metabolic disorders. Recent report indicated Mulberry leaf extract (MLE) attenuated dyslipidemia and lipid accumulation in high fat diet (HFD)-fed mice. Non-alcoholic fatty liver (NAFLD) is generally considered as the liver component of metabolic syndrome. The hepatic lipid infiltration induces oxidative stress, and is associated with interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) which are regulated by the leptin and adiponectin. MLE could prevent obesity-related NAFLD via downregulating the lipogenesis enzymes while upregulating the lipolysis markers. Treatment of MLE, especially at 2%, enhanced the expression of superoxide dismutase (SOD) and clenched the oxidative stress of liver. MLE decreased the plasma level of leptin but increased adiponectin. The advantage of MLE is supposed mainly attributed to chlorogenic acid derivative. We suggest MLE, with promising outcome of research, could be nutraceutical to prevent obesity and related NAFLD.

Keywords: Adiponectin; Inflammation; Mulberry leaf; Non-alcoholic fatty liver; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipokines / metabolism*
  • Animals
  • Diet, High-Fat
  • Humans
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology
  • Lipogenesis / drug effects
  • Liver / drug effects
  • Liver / immunology
  • Liver / metabolism
  • Male
  • Morus / chemistry*
  • Non-alcoholic Fatty Liver Disease / etiology
  • Non-alcoholic Fatty Liver Disease / immunology
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Non-alcoholic Fatty Liver Disease / prevention & control*
  • Obesity / complications*
  • Oxidative Stress / drug effects*
  • Plant Extracts / administration & dosage*
  • Plant Leaves / chemistry
  • Rats, Wistar
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / immunology


  • Adipokines
  • Interleukin-6
  • Plant Extracts
  • Tumor Necrosis Factor-alpha

Grant support

This work was supported by a grant from the Ministry of Science and Technology, Taiwan (NSC 104-2811-B-040-007).