Induction of IL-10-balanced immune profiles following exposure to LTA from Staphylococcus epidermidis

Exp Dermatol. 2018 Apr;27(4):318-326. doi: 10.1111/exd.13540.


Staphylococcus epidermidis colonises human skin without apparent inflammation, but a dominance of S. epidermidis and S. aureus is characteristic of cutaneous microbial dysbiosis in atopic dermatitis (AD). While S. aureus can trigger AD, the role of S. epidermidis is less understood. We characterised consequences of innate immune sensing of lipoteichoic acid (LTA) preparations derived from S. epidermidis (epi-LTA) or S. aureus (aureus-LTA). Therefore, dendritic cell (DC) activation and consecutive priming of antigen-specific T cells following exposure of DC to epi-LTA or aureus-LTA were investigated. Mimicking acute AD, exposure of DC to IL-4 and LTAs was analysed. Exposure to epi-LTA or aureus-LTA activated human immune cells and murine dendritic cells (DCs) via TLR2/MyD88, however, resulting in divergent immune profiles. Differences between LTAs were significant for IL-6, IL-12p40 and IL-12p70 but not for IL-10, which was best reflected by the IL-12p70-to-IL-10 ratio being IL-10-balanced for epi-LTA but pro-inflammatory for aureus-LTA. LTA-exposed DCs activated CD4+ T cells; however, while T-cell-derived IL-10 was equivalent between LTAs, IFN-γ and IL-17 were significantly higher for aureus-LTA. Mimicking acute AD by exposing DCs to IL-4 and LTAs revealed that IL-4 significantly and uniformly suppressed epi-LTA-induced cytokine production, keeping the IL-12p70-to-IL-10 ratio balanced. In contrast, exposure of DCs to aureus-LTA and IL-4 enhanced IL-12p70 but suppressed IL-10 levels, further unbalancing the IL-12p70-to-IL-10 ratio. These data demonstrate opposing immune consequences following exposure to staphylococcal LTAs. Epi-LTA induced IL-10-balanced, aureus-LTA pro-inflammatory immune profiles.

Keywords: atopic dermatitis; cytokines; dendritic cells; lipoteichoic acid; microbial dysbiosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity / drug effects
  • Animals
  • Coculture Techniques
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Humans
  • Immunity, Innate / drug effects
  • Interleukin-10 / metabolism*
  • Interleukin-12 / metabolism
  • Interleukin-4 / pharmacology
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Lipopolysaccharides / pharmacology*
  • Mice
  • Myeloid Differentiation Factor 88 / metabolism
  • STAT6 Transcription Factor / metabolism
  • Staphylococcus aureus*
  • Staphylococcus epidermidis*
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / metabolism
  • Teichoic Acids / pharmacology*
  • Toll-Like Receptor 2 / metabolism
  • Tumor Necrosis Factor-alpha / metabolism


  • Interleukin-6
  • Interleukin-8
  • Lipopolysaccharides
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • STAT6 Transcription Factor
  • Stat6 protein, mouse
  • Teichoic Acids
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Tumor Necrosis Factor-alpha
  • interleukin-6, mouse
  • Interleukin-10
  • Interleukin-12
  • Interleukin-4
  • lipoteichoic acid