In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture

Robert M Hoffman; Aaron E Freeman

doi:10.1007/978-1-4939-7745-1_3

In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam^® Histoculture

Methods Mol Biol. 2018:1760:19-28. doi: 10.1007/978-1-4939-7745-1_3.

Authors

Robert M Hoffman^{1

2}, Aaron E Freeman³

Affiliations

¹ AntiCancer, Inc., San Diego, CA, USA. all@anticancer.com.
² Department of Surgery, University of California, San Diego, CA, USA. all@anticancer.com.
³ AntiCancer, Inc., San Diego, CA, USA.

PMID: 29572790
DOI: 10.1007/978-1-4939-7745-1_3

Abstract

Diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in 3-dimensional Gelfoam^® histoculture for long periods of time and still maintain many of their in vivo properties. The in vivo properties maintained in vitro include 3-dimensional growth; maintenance of tissue organization and structure, such as changes associated with oncogenic transformation; retention of differentiated function; tumorigenicity; and growth of multiple types of cells from a single tumor.

Keywords: 3-Dimensional; Gelfoam® histoculture; Tissue architecture function; Tumors.

MeSH terms

Animals
Biomarkers
Cell Culture Techniques*
Disease Models, Animal
Gene Expression
Heterografts
Histocytochemistry
Humans
Mice
Neoplasms / genetics
Neoplasms / metabolism
Neoplasms / pathology*
Tissue Culture Techniques*

Substances

Biomarkers