Comprehensive analysis of differentially expressed genes reveals the molecular response to elevated CO2 levels in two sea buckthorn cultivars

Guoyun Zhang; Tong Zhang; Juanjuan Liu; Jianguo Zhang; Caiyun He

doi:10.1016/j.gene.2018.03.057

Comprehensive analysis of differentially expressed genes reveals the molecular response to elevated CO₂ levels in two sea buckthorn cultivars

Gene. 2018 Jun 20:660:120-127. doi: 10.1016/j.gene.2018.03.057. Epub 2018 Mar 22.

Authors

Guoyun Zhang¹, Tong Zhang², Juanjuan Liu², Jianguo Zhang³, Caiyun He⁴

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China. Electronic address: zhangguoyun2014@gmail.com.
² State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
³ State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China; Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. Electronic address: zhangjg@caf.ac.cn.
⁴ State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China. Electronic address: hecy@caf.ac.cn.

PMID: 29574192
DOI: 10.1016/j.gene.2018.03.057

Abstract

Atmospheric carbon dioxide (CO₂) concentration increases every year. It is critical to understand the elevated CO₂ response molecular mechanisms of plants using genomic techniques. Hippophae rhamnoides L. is a high stress resistance plant species widely distributed in Europe and Asia. However, the molecular mechanism of elevated CO₂ response in H. rhamnoides has been limited. In this study, transcriptomic analysis of two sea buckthorn cultivars under different CO₂ concentrations was performed, based on the next-generation illumina sequencing platform and de novo assembly. We identified 4740 differentially expressed genes in sea buckthorn response to elevated CO₂ concentrations. According to the gene ontology (GO) results, photosystem I, photosynthesis and chloroplast thylakoid membrane were the main enriched terms in 'xiangyang' sea buckthorn. In 'zhongguo' sea buckthorn, photosynthesis was also the main significantly enriched term. However, the number of photosynthesis related differentially expressed genes were different between two sea buckthorn cultivars. Our GO and pathway analyses indicated that the expression levels of the transcription factors WRKY, MYB and NAC were significantly different between the two sea buckthorn cultivars. This study provides a reliable transcriptome sequence resource and is a valuable resource for genetic and genomic researches for plants under high CO₂ concentration in the future.

Keywords: CO(2) concentration; Differentially expressed genes; Molecular mechanism; Sea buckthorn; Transcriptome.

MeSH terms

Carbon Dioxide / metabolism*
Chloroplast Proteins / biosynthesis*
Chloroplast Proteins / genetics
Gene Expression Profiling
Gene Expression Regulation, Plant / physiology*
Hippophae / genetics
Hippophae / metabolism*

Substances

Chloroplast Proteins
Carbon Dioxide