Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair

Curr Cardiol Rep. 2018 Mar 24;20(5):35. doi: 10.1007/s11886-018-0979-6.


Purpose of review: Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts.

Recent findings: Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.

Keywords: Cardiovascular injury; Doxorubicin; Light-sheet imaging; Regeneration.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cardiovascular Diseases / diagnostic imaging*
  • Cardiovascular Diseases / pathology
  • Heart Injuries / diagnostic imaging*
  • Heart Injuries / pathology
  • Image Processing, Computer-Assisted*
  • Imaging, Three-Dimensional*
  • Microscopy, Fluorescence
  • Models, Animal
  • Models, Cardiovascular
  • Myocardium / pathology*
  • Regeneration / physiology*
  • Rodentia
  • Zebrafish / embryology*