Adenosine-loaded dissolving microneedle patches to improve skin wrinkles, dermal density, elasticity and hydration

Int J Cosmet Sci. 2018 Apr;40(2):199-206. doi: 10.1111/ics.12453.


Objective: Although dissolving microneedle patches have been widely studied in the cosmetics field, no comparisons have been drawn with the topical applications available for routine use. In this study, two wrinkle-improving products, adenosine-loaded dissolving microneedle patches and an adenosine cream, were evaluated for efficacy, with respect to skin wrinkling, dermal density, elasticity, and hydration, and safety in a clinical test on the crow's feet area.

Methods: Clinical efficacy and safety tests were performed for 10 weeks on 22 female subjects with wrinkles around their eyes. The adenosine-loaded dissolving microneedle patch was applied once every 3 days, in the evening, for 8 weeks to the designated crow's feet area. The adenosine cream was applied two times per day, in the morning and evening, for 8 weeks to the other crow's feet area. Skin wrinkling, dermal density, elasticity, and hydration were measured by using PRIMOS® premium, Dermascan® C, Cutometer® MPA580, and Corneometer® CM 825, respectively. In addition, subjective skin irritation was evaluated by self-observation, and objective skin irritation was assessed through expert interviews.

Results: The adenosine-loaded dissolving microneedle patches had a similar or better efficacy than the adenosine cream. Both groups showed statistically significant efficacy for almost all parameters (P < 0.05). The dissolving microneedle patches had a long-lasting effect on the average wrinkle depth (P < 0.05), only showed efficacy in dermal density (P < 0.05), had an early improving effect on elasticity (P < 0.05), and demonstrated better hydration efficacy (P < 0.001). No adverse effects were observed in either group during the test period.

Conclusions: In the clinical efficacy test of four skin-improvement parameters, adenosine-loaded dissolving microneedle patches showed the same or better effect than the adenosine cream, although the weekly adenosine dose was 140 times lower. The dissolving microneedle patches caused no adverse reactions. These adenosine-loaded dissolving microneedle patches are expected to be safe, effective, and novel cosmetics for skin improvement.

Keywords: delivery/vectorisation/penetration; dissolving microneedle; safety testing; skin physiology/structure; wrinkle improvement.

Publication types

  • Clinical Study
  • Comparative Study

MeSH terms

  • Adenosine / administration & dosage*
  • Administration, Cutaneous
  • Adult
  • Animals
  • Cosmetic Techniques* / adverse effects
  • Elasticity
  • Female
  • Humans
  • Middle Aged
  • Skin Aging*
  • Skin Cream / administration & dosage
  • Swine
  • Transdermal Patch* / adverse effects
  • Treatment Outcome
  • Water


  • Water
  • Adenosine