Wheat expansin gene TaEXPA2 is involved in conferring plant tolerance to Cd toxicity

Abstract

Cadmium (Cd) is a severe and toxic heavy metal pollutant that affects plant growth and development. In this study, we found that the expression of an expansin gene, TaEXPA2, was upregulated in wheat leaves under CdCl₂ toxicity. We characterized the involvement of TaEXPA2 in conferring Cd tolerance. Tobacco plants overexpressing TaEXPA2 showed higher germination rate, root elongation, and biomass accumulation compared to the wild-type (WT) plants upon CdCl₂ treatment. The improved photosynthetic parameters and lesser cellular damage in transgenic plants exposed to Cd compared to that in the WT plants suggest that TaEXPA2 overexpression improves Cd tolerance in plants. Furthermore, we noticed that Cd was efficiently effluxed out of the cytoplasm in the transgenic plants owing to the enhanced activities of H⁺-ATPase, V-ATPase, and PPase, which helped in conferring Cd tolerance. Moreover, Cd concentration and ROS accumulation were lower in the transgenic plants than in WT plants as a consequence of enhanced antioxidant enzyme activities in the former. In addition, atexpa2, an Arabidopsis mutant, exhibited lower biomass and shorter primary root compared to its WT under Cd toxicity; however, the phenotype was recovered upon expression of TaEXPA2 in these mutants. Our results demonstrate that TaEXPA2 confers tolerance to Cd toxicity. The changed absorption/transportation of Cd and the antioxidative capacity may be involved in the improved tolerance of the transgenic plants with overexpression of TaEXPA2 to CdCl₂ toxicity.

Keywords: Cd efflux; Cd toxicity; Cell damage; Expansin; Transgenic plants; Wheat.