Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital

Fanhong Kong; Wenli Zhang; Lin Qiao; Qi Li; Haowen Li; Jingli Cao; Wenyan He; Chengya Dong; Yanjiao He; Lu He; Li Liu; Weilun Fu; Lijun Liu; Zirui Li; Yajie Wang

doi:10.7717/peerj.4450

Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital

PeerJ. 2018 Mar 13:6:e4450. doi: 10.7717/peerj.4450. eCollection 2018.

Authors

Fanhong Kong^#¹, Wenli Zhang^#¹, Lin Qiao², Qi Li², Haowen Li², Jingli Cao², Wenyan He², Chengya Dong², Yanjiao He³, Lu He¹, Li Liu², Weilun Fu¹, Lijun Liu⁴, Zirui Li², Yajie Wang⁵

Affiliations

¹ Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
² Clinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
³ Neuropathological department, Beijing Neurosugical Institute, Capital Medical University, Beijing, China.
⁴ Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
⁵ Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

^# Contributed equally.

Abstract

Background: We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures.

Methods: One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination.

Results: The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation.

Conclusion: Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.

Keywords: Biobank; Glioma; Quality evaluation.

Grants and funding

This study was supported by the Natural Science Foundation of China (nos. 81572474), and the Science and Technology Development Fund Project of Traditional Chinese Medicine of Beijing (JJ2015-14). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.