Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec;7(6):e00607.
doi: 10.1002/mbo3.607. Epub 2018 Mar 25.

Susceptibilities of Candidatus Liberibacter asiaticus-infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatures

Mubasher Hussain  1   2   3   4 , Komivi Senyo Akutse  1   2   4   5 , Yongwen Lin  1   2   4 , Shiman Chen  1   2   4 , Wei Huang  1   2   4 , Jinguan Zhang  1   2   4 , Atif Idrees  1   6 , Dongliang Qiu  3 , Liande Wang  1   2   4
Affiliations

Susceptibilities of Candidatus Liberibacter asiaticus-infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatures

Mubasher Hussain et al. Microbiologyopen. 2018 Dec.

Abstract

Some entomopathogenic fungi species, Isaria fumosorosea, and Hirsutella citriformis were found to be efficient against the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). However, the susceptibility to these fungi increases when the psyllid infected with Candidatus Liberibacter asiaticus (Las), which is transmitted by D. citri and causes citrus greening disease. In this study, we examined the Las-infected and Las-uninfected D. citri susceptibility to entomopathogenic fungi at different temperature regimes (5-40°C). When D. citri adults exposed to cold temperature (5°C), they showed less susceptibility to entomopathogenic fungi as compared with control (27°C). Irrespective of infection with Las, a significantly positive correlation was observed between temperature and percentage mortality caused by different isolates of I. fumosorosea, 3A Ifr, 5F Ifr, PS Ifr, and H. citriformis isolates, HC3D and 2H. In contrast, a significantly negative correlation was found between temperature and percentage mortality for 3A Ifr for both Las-infected and Las-uninfected psyllids. Detoxification enzymes, Glutathione S-transferase levels in D. citri showed a negative correlation, whereas cytochrome P450 and general esterase levels were not correlated with changes in temperature. These findings revealed that detoxification enzymes and general esterase levels are not correlated with altered susceptibility to entomopathogenic fungi at the different temperature regimes. Conclusively, temperature fluctuations tested appear to be a significant factor impacting the management strategies of D. citri using entomopathogenic fungi.

Keywords: asian citrus psyllid; bacterial infection; detoxification enzymes; entomopathogenic fungi; microbial ecology; temperature effects.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Cytochrome P450 (a), general esterase (b) and glutathione S‐transferase (c) activity levels comparison in Las‐uninfected Diaphorina citri at different temperatures regimes. Bars represent means with standard errors (SE), and means followed by different letters indicate a significant difference at p < 0.05
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Abbott, W. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18, 265–267. - PubMed
    1. Avery, P. B. , Pick, D. A. , Aristizabal, L. F. , Kerrigan, J. , Powell, C. A. , Rogers, M. E. , & Arthurs, S. P. (2013). Compatibility of Isaria fumosorosea (Hypocreales: Cordycipitaceae) Blastospores with agricultural chemicals used for management of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae). Insects, 4, 694–711. - PMC - PubMed
    1. Avery, P. B. , Wekesa, V. W. , Hunter, W. B. , Hall, D. G. , McKenzie, C. L. , Osborne, L. S. , … Rogers, M. E. (2011). Effects of the fungus Isaria fumosorosea (Hypocreales: Cordycipitaceae) on reduced feeding and mortality of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae). Biocontrol Science and Technology, 21, 1065–1078.
    1. Boina, D. R. , Onagbola, E. O. , Salyani, M. , & Stelinski, L. L. (2009). Influence of posttreatment temperature on the toxicity of insecticides against Diaphorina citri (Hemiptera: Psyllidae). Journal of Economic Entomology, 102, 685–691. - PubMed
    1. Bove, J. M. , & Ayres, A. J. (2007). Etiology of three recent diseases of citrus in Sao Paulo State: Sudden death, variegated chlorosis and huanglongbing. IUBMB Life, 59, 346–354. - PubMed

Publication types

LinkOut - more resources