Rapid determination of the aromatic compounds methyl-anthranilate, 2'-aminoacetophenone and furaneol by GC-MS: Method validation and characterization of grape derivatives

Food Res Int. 2018 May;107:613-618. doi: 10.1016/j.foodres.2018.03.020. Epub 2018 Mar 5.


A methodology for the rapid determination of the aromatic compounds methyl anthranilate (MA), 2'-aminoacetophenone (2-AAP) and furaneol by GC-MS was validated and used to characterize grape juice and wine elaborated with the new Brazilian grape varieties cultivated in northeastern Brazil, and Brazilian grape nectars. The method presented linearity (R2 ˃ 0.9952), good accuracy (CV < 12.9%), recovery (76.6% to 106.3%), limit of detection (23 μg L-1 to 94 μg L-1) and limit of quantification (96 μg L-1 to 277 μg L-1) acceptable in only 20 min of running. The methodology was considered satisfactory for the purpose, being a simple and rapid method for the determination of these compounds in grape derivatives drinks. In the characterization of the nectars the compound that stood out was the MA, being its presence attributed to the addition of flavorings in these products. It was evidenced a significant contribution of furaneol in the aroma of grape juice and wines elaborated with the new Brazilian grape varieties.

Keywords: Beverages; Food analysis; Hybrid grapes; Quality control; Vitis labrusca L.; Volatile compounds.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acetophenones / analysis*
  • Brazil
  • Calibration
  • Food Analysis / methods*
  • Food Analysis / standards
  • Fruit and Vegetable Juices / analysis*
  • Furans / analysis*
  • Gas Chromatography-Mass Spectrometry* / standards
  • Limit of Detection
  • Odorants / analysis*
  • Reference Standards
  • Reproducibility of Results
  • Smell*
  • Time Factors
  • Vitis / chemistry*
  • Vitis / growth & development
  • Wine / analysis*
  • Workflow
  • ortho-Aminobenzoates / analysis*


  • Acetophenones
  • Furans
  • ortho-Aminobenzoates
  • furaneol
  • 2-aminoacetophenone
  • methyl anthranilate